Advances in the Development of Gene-Targeting Vectors to Increase the Efficiency of Genetic Modification

• Saito Shinta

  Graduate School of Nanobioscience, Yokohama City University

• Adachi Noritaka

  Graduate School of Nanobioscience, Yokohama City University Advanced Medical Research Center, Yokohama City University

抄録

Gene targeting via homologous recombination, albeit highly inefficient in human cells, is considered a powerful tool for analyzing gene functions. Despite recent progress in the application of artificial nucleases for genome editing, safety issues remain a concern, particularly when genetic modification is used for therapeutic purposes. Therefore, the development of gene-targeting vectors is necessary for safe and sophisticated genetic modification. In this paper, we describe the effect of vector structure on random integration, which is a major obstacle in efficient gene targeting. In addition, we focus on the features of exon-trapping-type gene-targeting vectors, and discuss a novel strategy for negative selection to enhance gene targeting in human cells.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 39 (1), 25-32, 2016

  公益社団法人 日本薬学会

キーワード

• gene targeting
• targeting vector
• random integration
• exon-trap

詳細情報

• CRID: 1390001204632351744
• NII論文ID: 130005116505
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b15-00701
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 027012978
• PubMed: 26725425
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027012978
• Web Site: https://www.jstage.jst.go.jp/article/bpb/39/1/39_b15-00701/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016233218
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
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  • KAKEN
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