Advances in the Development of Gene-Targeting Vectors to Increase the Efficiency of Genetic Modification

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Author(s)

Abstract

Gene targeting <i>via</i> homologous recombination, albeit highly inefficient in human cells, is considered a powerful tool for analyzing gene functions. Despite recent progress in the application of artificial nucleases for genome editing, safety issues remain a concern, particularly when genetic modification is used for therapeutic purposes. Therefore, the development of gene-targeting vectors is necessary for safe and sophisticated genetic modification. In this paper, we describe the effect of vector structure on random integration, which is a major obstacle in efficient gene targeting. In addition, we focus on the features of exon-trapping-type gene-targeting vectors, and discuss a novel strategy for negative selection to enhance gene targeting in human cells.

Journal

  • Biological and Pharmaceutical Bulletin

    Biological and Pharmaceutical Bulletin 39(1), 25-32, 2016

    The Pharmaceutical Society of Japan

Codes

  • NII Article ID (NAID)
    130005116505
  • NII NACSIS-CAT ID (NCID)
    AA10885497
  • Text Lang
    ENG
  • ISSN
    0918-6158
  • NDL Article ID
    027012978
  • NDL Call No.
    Z53-V41
  • Data Source
    NDL  J-STAGE 
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