Robust Circadian Rhythm and Parathyroid Hormone-Induced Resetting during Hypertrophic Differentiation in ATDC5 Chondroprogenitor Cells

• Hosokawa Toshihiro

  Department of Physiology and Systems Bioscience, Kyoto Prefectural University of Medicine Department of Musculoskeletal Chronobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

• Tsuchiya Yoshiki

  Department of Physiology and Systems Bioscience, Kyoto Prefectural University of Medicine

• Okubo Naoki

  Department of Physiology and Systems Bioscience, Kyoto Prefectural University of Medicine Department of Musculoskeletal Chronobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

• Kunimoto Tatsuya

  Department of Physiology and Systems Bioscience, Kyoto Prefectural University of Medicine Department of Musculoskeletal Chronobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

• Minami Yoichi

  Department of Physiology and Systems Bioscience, Kyoto Prefectural University of Medicine Department of Musculoskeletal Chronobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

• Fujiwara Hiroyoshi

  Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

• Umemura Yasuhiro

  Department of Physiology and Systems Bioscience, Kyoto Prefectural University of Medicine

• Koike Nobuya

  Department of Physiology and Systems Bioscience, Kyoto Prefectural University of Medicine

• Kubo Toshikazu

  Department of Musculoskeletal Chronobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

• Yagita Kazuhiro

  Department of Physiology and Systems Bioscience, Kyoto Prefectural University of Medicine Department of Musculoskeletal Chronobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

抄録

Cartilage tissues possess intrinsic circadian oscillators, which influence chondrocyte function and chondrocyte specific gene expression. However, it is not fully understood how chondrogenesis influences the circadian clock, and vice versa. Thus, we established ATDC5 cells which were stably transfected with the Bmal1:luc reporter and revealed robust circadian rhythms in ATDC5 cells during differentiation. Moreover, the circadian clock in ATDC5 cells was strongly reset by PTH in a circadian time-dependent manner. This assay system is expected to be useful for investigating the role of the circadian clock in chondrogenic differentiation and the precise molecular mechanisms underlying PTH action on the chondrocyte circadian clock.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 48 (6), 165-171, 2015

  日本組織細胞化学会

キーワード

• circadian rhythm
• chondrogenic differentiation
• ATDC5
• parathyroid hormone

詳細情報

• CRID: 1390001204861566976
• NII論文ID: 130005116781
• DOI: 10.1267/ahc.15025
• ISSN: 13475800
• ISSN: 00445991
• Web Site: https://www.jstage.jst.go.jp/article/ahc/48/6/48_15025/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016310912
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可