Visualization of Kisspeptin Binding to Rat Hypothalamic Neurons

• Iijima Norio

  Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School

• Takumi Ken

  Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School

• Matsumoto Keisuke

  Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School

• Ozawa Hitoshi

  Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School

Abstract

The neuropeptide kisspeptin plays an important role in fertility and the onset of puberty, stimulating gonadotropin-releasing hormone (GnRH) neurons to activate the hypothalamic–pituitary–gonadal axis. Several studies have demonstrated a morphological interaction between kisspeptin- and GnRH-expressing neurons; however, few have addressed the interaction of kisspeptin with other neuronal subtypes. We recently showed that fibers immunoreactive for kisspeptin were densely distributed in the dorsal part of the arcuate nucleus. These fibers were found in close proximity to GnRH and tuberoinfundibular dopamine (TIDA) neurons. In the present study, using biotinylated kisspeptin, we established a visualization method for identifying kisspeptin binding sites on TIDA neurons. Biotinylated kisspeptin bound to the cell bodies of TIDA neurons and surrounding fibers, suggesting that TIDA neurons express sites of action for kisspeptin. Our assay also detected biotinylation signals from kisspeptin binding to GnRH fibers in the median eminence, but not to cell bodies of GnRH neurons in the medial preoptic area. Positive signals were completely eliminated by addition of excess non-labeled kisspeptin. This method enabled us to detect kisspeptin binding sites on specific neural structures and neuronal fibers.

Journal

• ACTA HISTOCHEMICA ET CYTOCHEMICA

  ACTA HISTOCHEMICA ET CYTOCHEMICA 48 (6), 179-184, 2015

  JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

Keywords

• kisspeptin
• tuberoinfundibular dopaminergic neuron
• binding assay
• dopamine
• GnRH neuron

Details

• CRID: 1390001204861574400
• NII Article ID: 130005116784
• DOI: 10.1267/ahc.15017
• ISSN: 13475800
• ISSN: 00445991
• Web Site: https://www.jstage.jst.go.jp/article/ahc/48/6/48_15017/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016310914
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed