Maintenance of the Extracellular Matrix in Rat Anterior Pituitary Gland: Identification of Cells Expressing Tissue Inhibitors of Metalloproteinases

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Author(s)

    • Azuma Morio
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Horiguchi Kotaro
    • Laboratory of Anatomy and Cell Biology, Department of Health Sciences, Kyorin University
    • Yashiro Takashi
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Tofrizal Alimuddin
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Maliza Rita
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Batchuluun Khongorzul
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Ramadhani Dini
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Syaidah Rahimi
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Tsukada Takehiro
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Fujiwara Ken
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Kikuchi Motoshi
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine|Laboratory of Natural History, Jichi Medical University School of Medicine

Abstract

The extracellular matrix (ECM) is important in creating cellular environments in tissues. Recent studies have demonstrated that ECM components are localized in anterior pituitary cells and affect cell activity. Thus, clarifying the mechanism responsible for ECM maintenance would improve understanding of gland function. Tissue inhibitors of metalloproteinases (TIMPs) are endogenous inhibitors of matrix metalloproteinases and participate in ECM degradation. In this study, we investigated whether cells expressing TIMPs are present in rat anterior pituitary gland. Reverse transcription polymerase chain reaction was used to analyze expression of the TIMP family (TIMP1-4), and cells producing TIMPs in the gland were identified by using <i>in situ</i> hybridization. Expression of TIMP1, TIMP2, and TIMP3 mRNAs was detected, and the TIMP-expressing cells were located in the gland. The TIMP-expressing cells were also investigated by means of double-staining with <i>in situ</i> hybridization and immunohistochemical techniques. Double-staining revealed that TIMP1 mRNA was expressed in folliculostellate cells. TIMP2 mRNA was detected in folliculostellate cells, prolactin cells, and thyroid-stimulating hormone cells. TIMP3 mRNA was identified in endothelial cells, pericytes, novel desmin-immunopositive perivascular cells, and folliculostellate cells. These findings indicate that TIMP1-, TIMP2-, and TIMP3-expressing cells are present in rat anterior pituitary gland and that they are involved in maintaining ECM components.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 48(6), 185-192, 2015

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

Codes

  • NII Article ID (NAID)
    130005116785
  • Text Lang
    ENG
  • ISSN
    0044-5991
  • Data Source
    J-STAGE 
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