A Simple Method for Measuring the Starch and Lipid Contents in the Cell of Microalgae

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著者

    • Takeshita Tsuyoshi
    • Department of Integrated Biosciences, Graduate school of Frontier Sciences, University of Tokyo
    • Takeda Kohei
    • Department of Integrated Biosciences, Graduate school of Frontier Sciences, University of Tokyo
    • Ota Shuhei
    • Department of Integrated Biosciences, Graduate school of Frontier Sciences, University of Tokyo|Japan Science and Technology Agency, CREST
    • Yamazaki Tomokazu
    • Department of Integrated Biosciences, Graduate school of Frontier Sciences, University of Tokyo|Japan Science and Technology Agency, CREST
    • Kawano Shigeyuki
    • Department of Integrated Biosciences, Graduate school of Frontier Sciences, University of Tokyo|Japan Science and Technology Agency, CREST

抄録

We used a microplate-based method to quantify microalgal lipids with Nile Red staining and a fluorescence microplate reader. However, a method to quantify starch that combines microplates with staining has not been reported. Therefore, we examined microplate-based quantification of lipids using Nile Red staining and of starch using Lugol staining. Neither starch nor lipids accumulated during the zero phase of cultured <i>Parachlorella kessleri</i>, only starch accumulated during the starch phase, and starch was subsequently lost and lipids accumulated during the oil phase. The quantities of starch and lipids were measured using a microplate-based method, which indicated linear production of starch and lipids within limited ranges (lipids, 0.071–0.380 g mL<sup>−1</sup>; starch, 155–404 mg mL<sup>-1</sup>) when standard curves were prepared for lipids extracted with methyl tertiary-butyl ether and for starch extracted with anthrone. The concentration of starch produced during the starch phase was 0.59 mg mL<sup>−1</sup> and that of lipids during the oil phase was 2.49 mg mL<sup>−1</sup>. The concentration of starch produced was 0.05–0.13 mg mL<sup>−1</sup> during phases other than the starch phase, and lipids were not detected other than during the oil phase because lipid contents were approximated based on the quantity of triacylglycerol, which stains with Nile Red.

収録刊行物

  • CYTOLOGIA

    CYTOLOGIA 80(4), 475-481, 2015

    公益財団法人 日本メンデル協会

各種コード

  • NII論文ID(NAID)
    130005118533
  • 本文言語コード
    ENG
  • ISSN
    0011-4545
  • データ提供元
    J-STAGE 
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