Sorcin Expression in the Epiphyseal Growth Plates of Mice

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Author(s)

    • Kawai Mariko
    • Department of Pharmacology, Osaka Dental University|Department of Oral Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
    • Liu Ning
    • Department of Oral Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences|Central Laboratory, The Second Hospital of Jilin University
    • Hattori Takako
    • Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
    • Kataoka Yo-Hei
    • Department of Oral Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
    • Takigawa Masaharu
    • Advanced Research Center for Oral and Craniofacial Sciences, Okayama University
    • Kubota Satoshi
    • Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
    • Yamamoto Toshio
    • Department of Oral Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

Abstract

Sorcin is a small calcium-binding protein that is widely expressed in many tissues. Sorcin regulates cardiac myocyte apoptosis by modulating mitochondrial Ca<sup>2+</sup> cycling and regulates fibroblast cell cycling and apoptosis via Ca<sup>2+ </sup>signaling through the endoplasmic reticulum (ER). During endochondral ossification, some chondrocytes undergo apoptosis after their terminal differentiation; however, Sorcin's expression in these cells has not been characterized. Here we examined Sorcin's gene expression in the chondrocytes derived from mouse growth plate by reverse transcription PCR (RT-PCR), and its protein localization in the chondrocytes of femoral growth plate using immunohistochemistry. Sorcin protein was detected in the chondrocytes, and was particularly abundant in the cytoplasm and nuclei of proliferative zone chondrocytes and in the nuclei of hypertrophic chondrocytes. Apoptotic analysis of the growth plate revealed that many of the hypertrophic chondrocytes undergo the DNA fragmentation. We report for the first time the localization of Sorcin in the epiphyseal growth plate in which one of the apoptotic phenomenon was detected.

Journal

  • Journal of Hard Tissue Biology

    Journal of Hard Tissue Biology 25(1), 57-62, 2016

    THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY

Codes

  • NII Article ID (NAID)
    130005119559
  • Text Lang
    ENG
  • ISSN
    1341-7649
  • Data Source
    J-STAGE 
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