Generation of Induced Pluripotent Stem Cells From Patients With Duchenne Muscular Dystrophy and Their Induction to Cardiomyocytes

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Author(s)

    • Hashimoto Akihito
    • Department of Cardiovascular Regenerative Medicine, Osaka University Graduate School of Medicine|Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • Nakagawa Akito
    • Department of Cardiovascular Regenerative Medicine, Osaka University Graduate School of Medicine|Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • Higo Tomoaki
    • Department of Cardiovascular Regenerative Medicine, Osaka University Graduate School of Medicine|Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • Kuramoto Yuki
    • Department of Cardiovascular Regenerative Medicine, Osaka University Graduate School of Medicine|Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • Sakai Taku
    • Department of Cardiovascular Regenerative Medicine, Osaka University Graduate School of Medicine|Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • Miyagawa Shigeru
    • Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine
    • Sawa Yoshiki
    • Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine
    • Naito Atsuhiko T.
    • Department of Cardiovascular Regenerative Medicine, Osaka University Graduate School of Medicine|Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo
    • Sakata Yasushi
    • Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • Morita Hiroyuki
    • Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo
    • Umezawa Akihiro
    • Department of Reproductive Biology, Center for Regenerative Medicine, National Research Institute for Child Health and Development
    • Komuro Issei
    • Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo|Department of Institute for Academic Initiatives, Osaka University
    • Lee Jong-Kook
    • Department of Cardiovascular Regenerative Medicine, Osaka University Graduate School of Medicine|Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • Ito Masamichi
    • Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo
    • Yamaguchi Toshihiro
    • Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo
    • Nakata Ryo
    • Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo
    • Sumida Tomokazu
    • Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo
    • Okada Katsuki
    • Department of Cardiovascular Regenerative Medicine, Osaka University Graduate School of Medicine|Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine

Abstract

Duchenne muscular dystrophy (DMD) is caused by mutations in the <i>DMD</i> gene which encodes dystrophin protein. Dystrophin defect affects cardiac muscle as well as skeletal muscle. Cardiac dysfunction is observed in all patients with DMD over 18 years of age, but there is no curative treatment for DMD cardiomyopathy. To establish novel experimental platforms which reproduce the cardiac phenotype of DMD patients, here we established iPS cell lines from T lymphocytes donated from two DMD patients, with a protocol using Sendai virus vectors. We successfully conducted the differentiation of the DMD patient-specific iPS cells into beating cardiomyocytes. DMD patient-specific iPS cells and iPS cell-derived cardiomyocytes would be a useful <i>in vitro</i> experimental system with which to investigate DMD cardiomyopathy.

Journal

  • International Heart Journal

    International Heart Journal 57(1), 112-117, 2016

    International Heart Journal Association

Codes

  • NII Article ID (NAID)
    130005120987
  • Text Lang
    ENG
  • ISSN
    1349-2365
  • Data Source
    J-STAGE 
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