Improvement and Interlaboratory Validation of the Lipophilic Oxygen Radical Absorbance Capacity: Determination of Antioxidant Capacities of Lipophilic Antioxidant Solutions and Food Extracts

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  • WATANABE Jun
    National Food Research Institute, National Agriculture and Food Research Organization
  • OKI Tomoyuki
    National Agricultural Research Center for Kyushu Okinawa Region, National Agriculture and Food Research Organization
  • TAKEBAYASHI Jun
    National Institutes of Biomedical Innovation, Health and Nutrition, National Institute of Health and Nutrition
  • YADA Hiroshi
    National Food Research Institute, National Agriculture and Food Research Organization
  • WAGAKI Manabu
    National Food Research Institute, National Agriculture and Food Research Organization
  • TAKANO-ISHIKAWA Yuko
    National Food Research Institute, National Agriculture and Food Research Organization
  • YASUI Akemi
    National Food Research Institute, National Agriculture and Food Research Organization

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抄録

A lipophilic oxygen radical absorbance capacity (L-ORAC) assay is an evaluation of the antioxidant capacity of solutions of lipophilic compounds. The concentrations of fluorescein, radical generator, and Trolox standard solutions were optimized to improve the precision of the assay. An interlaboratory study using two antioxidant solutions and three food extracts as test samples conducted in accordance with harmonized protocol demonstrated satisfactory L-ORAC measurements; the intermediate precision relative standard deviations (RSDint) ranged from 7.0 to 16.7%, the reproducibility relative standard deviations (RSDR) ranged from 14.8 to 19.4%, and the HorRat values ranged from 1.35 to 1.78.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 32 (2), 171-175, 2016

    社団法人 日本分析化学会

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