<b>P2Y purinoceptors mediate ATP-induced changes in intracellular calcium and amylase release in acinar cells of mouse parotid </b><b>glands </b>
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- MORIGUCHI-MORI Kasumi
- Department of Anatomy (Cell Biology), Iwate Medical University Division of Special Care Dentistry, Department of Developmental Oral Health Science, School of Dentistry, Iwate Medical University
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- HIGASHIO Hironori
- Department of Chemistry, Center for Liberal Arts and Sciences, Iwate Medical University
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- ISOBE Kanako
- Department of Anatomy (Cell Biology), Iwate Medical University Division of Special Care Dentistry, Department of Developmental Oral Health Science, School of Dentistry, Iwate Medical University
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- KUMAGAI Miho
- Division of Special Care Dentistry, Department of Developmental Oral Health Science, School of Dentistry, Iwate Medical University
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- SASAKI Kana
- Department of Anatomy (Cell Biology), Iwate Medical University
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- SATOH Yoh-ichi
- Department of Anatomy (Cell Biology), Iwate Medical University Department of Medical Education, Iwate Medical University
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- KUJI Akiyoshi
- Division of Special Care Dentistry, Department of Developmental Oral Health Science, School of Dentistry, Iwate Medical University
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- SAINO Tomoyuki
- Department of Anatomy (Cell Biology), Iwate Medical University
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Abstract
Adenosine 5’-triphosphate (ATP) can act as an extracellular signal that regulates various cellular functions. The present study aimed to determine which purinoceptors play a role in ATP-induced changes in intracellular Ca2+ ([Ca2+]i) and amylase secretion in mouse parotid glands. ATP induced a steep increase in [Ca2+]i in acinar cells. The removal of extracellular Ca2+ or the use of Ca2+ channel blockers slightly inhibited this increase. Inhibition of PLCγ by U73122 and of IP3 by xestospongin C did not completely block this increase. The purinoceptor antagonists suramin and reactive blue-2 strongly inhibited the ATP-induced changes in [Ca2+]i. 2-MeSATP induced a strong increase in [Ca2+]i, while Bz-ATP induced a small [Ca2+]i increase, and UTP and α,β-MeATP had no effect. The potency order of ATP analogs (2-MeSATP > ATP >> UTP) suggested that P2Y1 and P2Y12 play a significant role in the cellular response to ATP. RT-PCR revealed that P2X2,4,7 and P2Y1,2,10,12,14 were expressed in acinar cells. Ca2+-dependent exocytotic secretion of amylase was detected in parotid glands. These findings indicated that ATP activates P2Y receptors more than P2X receptors at low concentrations. Thus, P2Y receptors were found to be the main receptors involved in Ca2+-related cell homeostasis and amylase secretion in mouse parotid glands.
Journal
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- Biomedical Research
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Biomedical Research 37 (1), 37-49, 2016
Biomedical Research Press