Transient Receptor Potential Cation Channel Subfamily Vanilloid Member 3 is not Involved in Plasma Membrane Stretch-induced Intracellular Calcium Signaling in Merkel Cells

• Higashikawa Asuka

  Department of Physiology, Tokyo Dental College

• Kojima Yuki

  Department of Physiology, Tokyo Dental College

• Sato Masaki

  Department of Physiology, Tokyo Dental College

• Kimura Maki

  Department of Physiology, Tokyo Dental College

• Ogura Kazuhiro

  Department of Physiology, Tokyo Dental College

• Mochizuki Hiroyuki

  Department of Physiology, Tokyo Dental College

• Sase Toshiyuki

  Department of Crown and Bridge Prosthodontics, Tokyo Dental College

• Shinya Akinori

  Department of Crown and Bridge Prosthodontics, Tokyo Dental College

• Kobune Kunio

  Department of Crown and Bridge Prosthodontics, Tokyo Dental College

• Furuya Tadashi

  Department of Crown and Bridge Prosthodontics, Tokyo Dental College

• Sato Toru

  Department of Crown and Bridge Prosthodontics, Tokyo Dental College

• Shibukawa Yoshiyuki

  Department of Physiology, Tokyo Dental College

• Tazaki Masakazu

  Department of Physiology, Tokyo Dental College

抄録

Merkel cells (MCs), which form part of the MC-neurite complex, making contact with sensory afferents to drive mechanosensory transduction mechanisms, express transient receptor potential (TRP) cation channel subfamily vanilloid (V) members 1, 2, and 4, as well as ankyrin subfamily member 1. While these proteins are involved in sensing plasma membrane stretch, less is known about the functional properties of TRPV subfamily member 3 (TRPV3) during membrane stretch in MCs. The aim of this study was to determine whether TRPV3 channels were involved in mechanosensory activity by measuring intracellular free Ca²⁺ concentrations ([Ca²⁺]_i) in MCs isolated from hamster buccal mucosa. Application of a hypotonic extracellular solution to quinacrine-positive MCs elicited a transient increase in [Ca²⁺]_i. When TRPV3 channel antagonist 2,2-diphenyltetrahydrofuran was added to the hypotonic extracellular solution, however, no effect was observed on hypotonic stimulation-induced increase in [Ca²⁺]_i. These results suggest that TRPV3 channels are not involved in the mechanosensory mechanism during membrane stretch in MCs.

収録刊行物

• The Bulletin of Tokyo Dental College

  The Bulletin of Tokyo Dental College 56 (4), 259-262, 2015

  東京歯科大学

キーワード

• Merkel cell
• TRP channel
• Mechanotransduction
• Sensory transduction

詳細情報

• CRID: 1390282679565476864
• NII論文ID: 130005129286
• NII書誌ID: AA00592969
• DOI: 10.2209/tdcpublication.56.259
• ISSN: 00408891
• PubMed: 26657525
• Web Site: https://www.jstage.jst.go.jp/article/tdcpublication/56/4/56_259/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016292813
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可