Distribution and toxicity evaluation of ZnO dispersion nanoparticles in single intravenously exposed mice

• Fujihara Junko

  Department of Legal Medicine, Shimane University Faculty of Medicine

• Tongu Miki

  Shin-yamanote Hospital, Higashi-murayama

• Hashimoto Hideki

  Center for the Promotion of Project Research, Shimane University

• Yamada Takaya

  Department of Experimental Animals, Center for Integrated Research in Science, Shimane University Faculty of Medicine

• Kimura-Kataoka Kaori

  Department of Legal Medicine, Shimane University Faculty of Medicine

• Yasuda Toshihiro

  Division of Medical Genetics and Biochemistry, Faculty of Medical Sciences, University of Fukui

• Fujita Yasuhisa

  Interdisciplinary Graduate School of Science and Engineering, Shimane University

• Takeshita Haruo

  Department of Legal Medicine, Shimane University Faculty of Medicine

Abstract

ZnO nanoparticles (NPs) have been widely used in various commercial products. Application of ZnO NPs is expected to apply to cancer diagnosis and therapy, used as drug delivery carriers. In the present study, the lethal dose 50 (LD₅₀) of intravenously administered ZnO NPs (0.3 mg/kg) was calculated in mice. Blood kinetics and tissue distribution of a toxic dose of ZnO NPs (0.2 mg/kg, 0.05 mg/kg) were investigated after intravenous exposure. In addition, 8-hydroxy-2'-deoxyguanosine (8-OHdG) was evaluated. Following the injection, ZnO NPs were rapidly removed from the blood and distributed to organs. Pulmonary emphysema was observed pathologically study in mice at 3 days after the 0.2 mg/kg dose and at 6 days after the 0.05 mg/kg dose. ZnO NPs were mainly accumulated in the lung and spleen within 60 min. From the long-term tissue distribution study, the liver showed peak concentration at 6 days, and spleen peaked at 1 day. The lungs kept high levels until 6 days. Tissue distribution and pathological study showed that the spleen, liver, and lungs are target organs for ZnO NPs. Accumulation in the liver and spleen may be due to the phagocytosis by macrophages. A dose-dependent increase in 8-OHdG was observed in mice treated with ZnO NPs. This study is the first to show information on kinetics and target organs following intravenous ZnO injection. J. Med. Invest. 62: 45-50, February, 2015

Journal

• The Journal of Medical Investigation

  The Journal of Medical Investigation 62 (1.2), 45-50, 2015

  The University of Tokushima Faculty of Medicine

Keywords

• Nanoparticles
• Intravenous injection
• ZnO
• Acute toxicity
• Tissue distribution

Details

• CRID: 1390282679221670784
• NII Article ID: 130005129687
• NII Book ID: AA11166929
• DOI: 10.2152/jmi.62.45
• ISSN: 13496867
• ISSN: 13431420
• PubMed: 25817283
• Web Site: https://repo.lib.tokushima-u.ac.jp/111245
• Web Site: https://www.jstage.jst.go.jp/article/jmi/62/1.2/62_45/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016077520
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed