Direct Lipido-Metabolomics of Single Floating Cells for Analysis of Circulating Tumor Cells by Live Single-cell Mass Spectrometry

• HIYAMA Eiso

  Natural Science Center for Basic Research and Development, Hiroshima University

• ALI Ahmed

  Faculty of Pharmacy, Misr International University Quantitative Biology Center (QBiC), RIKEN

• AMER Sara

  Faculty of Pharmacy, Misr International University Quantitative Biology Center (QBiC), RIKEN

• HARADA Takanori

  Natural Science Center for Basic Research and Development, Hiroshima University

• SHIMAMOTO Kazumi

  Natural Science Center for Basic Research and Development, Hiroshima University

• FURUSHIMA Rie

  Quantitative Biology Center (QBiC), RIKEN

• ABOULEILA Yasmine

  Faculty of Pharmacy, Misr International University Quantitative Biology Center (QBiC), RIKEN

• EMARA Samy

  Faculty of Pharmacy, Misr International University

• MASUJIMA Tsutomu

  Quantitative Biology Center (QBiC), RIKEN

Abstract

Direct trapping of a single floating cell, i.e. a white blood cell from a drop of blood, within a nanospray tip was followed by super-sonication after the addition of ionization solvent. Molecular detection of an increased number of peaks with a higher intensity and a wider m/z range, which extends from metabolites to lipids, was acquired than of that without sonication. This method was applied to a few separated circulating tumor cells (CTC) from a neuroblastoma patient’s blood to obtain their lipido-metabolomic molecular profile at the single cell level. In addition to vital molecules such as amino acids, catechol amine metabolites, which are specific to neuroblastoma, and drugs included in the patient’s course of therapy were detected. This established “direct single-cell lipido-metabolomic method” seems to be useful for direct and wide range molecular detection not only for many live single-cells, but also for rare cells, such as CTCs, for future molecular diagnosis.

Journal

• Analytical Sciences

  Analytical Sciences 31 (12), 1215-1217, 2015

  The Japan Society for Analytical Chemistry

Keywords

• Live single-cell mass spectrometry
• circulating tumor cell
• neuroblastoma
• floating cells
• lipido-metabolomics
• one-drop blood analysis

Details

• CRID: 1390282679238184832
• NII Article ID: 130005129718
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.31.1215
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 027106620
• PubMed: 26656808
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027106620
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.31.1215.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.31.1215/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/31/12/31_1215/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016219463
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed