Mass Spectrometric Quantification of Amphipathic, Polyphenolic Antioxidant of the Pacific Oyster (<i>Crassostrea Gigas</i>)
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A novel amphipathic phenolic compound, 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), that can be isolated from the Pacific oyster (<i>Crassostrea gigas</i>) has been found to protect human hepatocytes against oxidative stress. This study aims to establish a method for the measurement of DHMBA for industrial application. Liquid chromatography–tandem mass spectrometry using deuterated DHMBA as an internal standard and a polar end-capped ODS (Hypersil GOLD aQ) as the solid phase was validated. The limit of detection was 0.04 pmol (<i>S/N</i> = 5), and the limit of quantitation was 0.1 pmol (<i>S/N</i> = 10). The calibration curve was linear throughout the range of 0.1 – 16 pmol (<i>r</i><sup>2</sup> = 0.9995). This method successfully quantified DHMBA in oysters from 11 sea areas in Japan. The results showed that the yield of DHMBA was variable from 9.8 to 58.8 μg g<sup>−1</sup> whole oyster meat wet weight but not affected by the seawater temperature. The proposed LC-MS/MS method is useful in quantitative studies for DHMBA and potentially for other amphipathic substances.
- Analytical Sciences
Analytical Sciences 31(12), 1341-1344, 2015
Japan Society for Analytical Chemistry