Mass Spectrometric Quantification of Amphipathic, Polyphenolic Antioxidant of the Pacific Oyster (Crassostrea Gigas)

• OKABE Hiroaki

  Faculty of Health Sciences, Hokkaido University

• HUI Shu-Ping

  Faculty of Health Sciences, Hokkaido University

• FUDA Hirotoshi

  Faculty of Health Sciences, Hokkaido University

• FURUKAWA Takayuki

  Faculty of Health Sciences, Hokkaido University

• TAKEDA Seiji

  Faculty of Health Sciences, Hokkaido University

• SHRESTHA Rojeet

  Faculty of Health Sciences, Hokkaido University

• MIURA Yusuke

  Faculty of Health Sciences, Hokkaido University

• WATANABE Mitsugu

  Watanabe Oyster Laboratory Co., Ltd.

• CHIBA Hitoshi

  Faculty of Health Sciences, Hokkaido University

書誌事項

タイトル別名

• Mass Spectrometric Quantification of Amphipathic, Polyphenolic Antioxidant of the Pacific Oyster (<i>Crassostrea Gigas</i>)

抄録

A novel amphipathic phenolic compound, 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), that can be isolated from the Pacific oyster (Crassostrea gigas) has been found to protect human hepatocytes against oxidative stress. This study aims to establish a method for the measurement of DHMBA for industrial application. Liquid chromatography–tandem mass spectrometry using deuterated DHMBA as an internal standard and a polar end-capped ODS (Hypersil GOLD aQ) as the solid phase was validated. The limit of detection was 0.04 pmol (S/N = 5), and the limit of quantitation was 0.1 pmol (S/N = 10). The calibration curve was linear throughout the range of 0.1 – 16 pmol (r² = 0.9995). This method successfully quantified DHMBA in oysters from 11 sea areas in Japan. The results showed that the yield of DHMBA was variable from 9.8 to 58.8 μg g⁻¹ whole oyster meat wet weight but not affected by the seawater temperature. The proposed LC-MS/MS method is useful in quantitative studies for DHMBA and potentially for other amphipathic substances.

収録刊行物

• Analytical Sciences

  Analytical Sciences 31 (12), 1341-1344, 2015

  社団法人 日本分析化学会

キーワード

• Amphipathicity
• polyphenol
• LC-MS/MS
• antioxidant
• Pacific oyster
• polar end-capped ODS

詳細情報

• CRID: 1390001204261554176
• NII論文ID: 130005129739
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.31.1341
• ISSN: 13482246
• ISSN: 09106340
• HANDLE: 2115/62970
• NDL書誌ID: 027106847
• PubMed: 26656828
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027106847
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.31.1341.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.31.1341/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/31/12/31_1341/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016219474
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可