The Effect of Artificial Recombinant Bone Morphogenetic Protein 2 with Titanium-binding Peptide on a Ti Graft <i>in Vivo</i>

DOI
  • KOKUBU Eitoyo
    Oral Health Science Center, and Department of Microbiology, Tokyo Dental College, Tokyo, Japan
  • KOKUBUN Katsutoshi
    Oral Health Science Center, and Department of Clinical Pathophysiology, Tokyo Dental College, Tokyo, Japan
  • MATSUZAKA Kenichi
    Oral Health Science Center, and Department of Clinical Pathophysiology, Tokyo Dental College, Tokyo, Japan
  • KASHIWAGI Kenji
    Division of Protein Engineering. The Cancer Institute of the Japanese foundation for Cancer Research, Tokyo, Japan
  • SHIBA Kiyotaka
    Division of Protein Engineering. The Cancer Institute of the Japanese foundation for Cancer Research, Tokyo, Japan
  • INOUE Takashi
    Oral Health Science Center, and Department of Clinical Pathophysiology, Tokyo Dental College, Tokyo, Japan
  • ISHIHARA Kazuyuki
    Oral Health Science Center, and Department of Microbiology, Tokyo Dental College, Tokyo, Japan
  • YOSHINARI Masao
    Oral Health Science Center, and Department of Dental Materials Science, Tokyo Dental College, Tokyo, Japan

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Surface modification of titanium (Ti) influences cell behavior, and immobilization of bone morphogenetic protein (BMP)-2 on a Ti surface cells to differentiate into osteogenic cells. This study aimed to evaluate the effect of an artificial fusion protein (AFP) comprising a modified BMP-2 and Ti-binding peptide (TBP) motif (AFP-TBP–BMP-2) on osteogenesis of rat abdominal cells on Ti surface in vivo. Ti plates were dipped in three different mixtures of collagen gel: collagen gel only, collagen gel with BMP-2, and collagen gel with AFP-TBP–BMP-2. The treated Ti plates were then implanted into rat abdominal muscles. One and 2 weeks after implantation, these plates were histologically observed. The Ti plate with collagen gel and AFP-TBP–BMP-2 produced cartilage in the muscle at 1 week, and bone-like hard tissue was observed at 2 weeks. These results suggest that the application of collagen gel with AFP-TBP–BMP-2 accelerates osteogenesis in vivo.

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