Interactome analysis reveals molecular mechanisms underlying the association between selenium binding protein 1 expression and the malignant features of tumor cells

• Tajima Takashi

  Division of Rare Cancer Research, National Cancer Center Research Institute

• Kito Fusako

  Division of Rare Cancer Research, National Cancer Center Research Institute

• Ohta Tsutomu

  Division of Rare Cancer Research, National Cancer Center Research Institute

• Shiozawa Kumiko

  Division of Rare Cancer Research, National Cancer Center Research Institute

• Kawai Akira

  Division of Musculoskeletal Oncology, National Cancer Center Hospital

• Kondo Tadashi

  Division of Rare Cancer Research, National Cancer Center Research Institute

抄録

The potential biological and clinical significance of selenium binding protein 1 (SBP1) has been suggested in various types of cancer. To evaluate the role of SBP1 and reveal the molecular basis for its function, we examined the SBP1 protein complex. A gene transfection assay revealed that overexpression of SBP1 promoted proliferation and migration of A549 lung adenocarcinoma cells. Halo-tag-based affinity purification coupled with liquid chromatography-tandem mass spectrometry identified 23 components of the SBP1 protein complex. The functional classification of these 23 proteins suggests that the SBP1 complex participates in critical biological events including cell structure, protein translation, stress response, chaperone, and apoptosis. Moreover, the SBP1 complex includes several proteins that are aberrantly expressed in cancers. These finding indicate that SBP1 may function coordinately with these multiple proteins to facilitate cancer progression. A comprehensive study of the multiple proteins associated with SPB1 together with an examination of individual proteins will be required to elucidate the roles of aberrant SBP1 regulation in cancer progression.

収録刊行物

• Journal of Electrophoresis

  Journal of Electrophoresis 59 (1), 1-6, 2015

  日本電気泳動学会

キーワード

• SBP1
• mass spectrometry
• proteome
• interactome

詳細情報

• CRID: 1390001205228595456
• NII論文ID: 130005130417
• DOI: 10.2198/jelectroph.59.1
• ISSN: 13499408
• ISSN: 13499394
• Web Site: https://www.jstage.jst.go.jp/article/jelectroph/59/1/59_1/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2017038106
• 本文言語コード: en
• データソース種別:
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