Tracking GFP-labeled Transplanted Mouse MSC in Nude Mice Using in Vivo Fluorescence Imaging

• TAIRA Masayuki

  Department of Biomedical Engineering, Iwate Medical University, Iwate, Japan

• HATAKEYAMA Wataru

  Department of Prosthodontics and Oral Implantology, Iwate Medical University School of Dentistry, Iwate, Japan

• YOKOTA Jun

  Department of Prosthodontics and Oral Implantology, Iwate Medical University School of Dentistry, Iwate, Japan

• CHOSA Naoyuki

  Division of Cellular Biosignal Sciences, Department of Biochemistry, Iwate Medical University, Iwate, Japan

• ISHISAKI Akira

  Division of Cellular Biosignal Sciences, Department of Biochemistry, Iwate Medical University, Iwate, Japan

• TAKAFUJI Kyoko

  Department of Prosthodontics and Oral Implantology, Iwate Medical University School of Dentistry, Iwate, Japan

• KIHARA Hidemichi

  Department of Prosthodontics and Oral Implantology, Iwate Medical University School of Dentistry, Iwate, Japan

• KONDO Hisatomo

  Department of Prosthodontics and Oral Implantology, Iwate Medical University School of Dentistry, Iwate, Japan

• HATTORI Masayuki

  Department of Biomedical Engineering, Iwate Medical University, Iwate, Japan

Abstract

The purpose of this study was to track the fate of GFP-labeled transgenic mouse mesenchymal stem cells (MSC) transplanted in nude mice. Mouse MSC had been collected from tibia of whole body GFP-labeled mice; were transplanted into five nude mice by tail vein injection; and were fed for 4 weeks. After comfort sacrifice, the existence and accumulation of GFP-labeled mouse MSC transplanted in nude mice was examined by in Vivo Florescence imaging. The searched cells were absent in bone marrow regions in dissected long bones (femurs). On the contrary, exposed abdominal cavities revealed that one out of five mice had a small fluorescent node on the liver surface. This means that at 20% probability, transplanted GFP-labeled mouse MSC could circulate in the vein, and settle and multiply in the terminal organ of a host mouse. This finding appears to contribute to future stem cell therapy by homing phenomenon of MSC.

Journal

• Nano Biomedicine

  Nano Biomedicine 6 (2), 73-77, 2014

  Nano Biomedical Society

Keywords

• tem cell therapy
• mesenchymal stem cells
• cell transplantation
• cell homing

Details

• CRID: 1390001205345809536
• NII Article ID: 130005130560
• DOI: 10.11344/nano.6.73
• ISSN: 21854734
• ISSN: 18835198
• Web Site: https://search.jamas.or.jp/link/ui/2015382692
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed