Theoretical Investigations into the Quantitative Mechanisms Underlying the Regulation of [cAMP]_i, Membrane Excitability and [Ca²⁺]_i during GLP-1 Stimulation in Pancreatic β Cells

• Takeda Yukari

  Department of Bioinformatics, College of Life Sciences, Ritsumeikan University

Bibliographic Information

Other Title

• 膵β細胞におけるGLP-1受容体刺激による細胞内cAMP濃度調節並びに膜興奮性及びカルシウム濃度制御機構のシミュレーション研究
• Symposium Review 膵β細胞におけるGLP-1受容体刺激による細胞内cAMP濃度調節並びに膜興奮性及びカルシウム濃度制御機構のシミュレーション研究
• Symposium Review スイvサイボウ ニ オケル GLP-1 ジュヨウタイ シゲキ ニ ヨル サイボウ ナイ cAMP ノウド チョウセツ ナラビニ マク コウフンセイ オヨビ カルシウム ノウド セイギョ キコウ ノ シミュレーション ケンキュウ

Abstract

  Upon elevation of plasma glucose concentration, pancreatic β-cells generate bursts of action potentials to induce cyclic changes in [Ca²⁺]_i regulating insulin release. Glucose-dependent insulin secretion is synergistically enhanced by glucagon-like peptide-1 (GLP-1), which increases [cAMP]_i and activates protein kinase A (PKA) and exchange protein activated by cAMP (Epac). The insulinotropic effect of GLP-1 is mediated, at least in part, by modulating multiple ion channels/transporters at the plasma membrane and ER through PKA- and EPAC-dependent mechanisms, which increase membrane excitability and intracellular Ca²⁺ release. However, because of complex interactions between multiple cellular factors involved in the GLP-1 effects, quantitative aspects of the molecular/ionic mechanisms have not yet been determined. We thus performed simulation studies and mathematical analysis to investigate how GLP-1 signals control [cAMP]_i and subsequently modify the bursting activities and Ca²⁺ dynamics. First, a GLP-1 receptor signal transduction model was developed and introduced to our β-cells model. Secondly, modulatory effects of PKA/Epac on ion channels/transporters were incorporated based on experimental studies. Increases in the frequency and duration of the bursting activity observed during GLP-1 stimulation were well reconstructed by our model, and lead potential analysis quantitatively determined the functional role of each ion channel/transporter in modifying the burst pattern. Finally, an IP₃R model was developed to reproduce GLP-1-induced Ca²⁺ transients/oscillations. Instantaneous equilibrium analysis and bifurcation analysis also elucidated the quantitative mechanisms involved in generating IP₃R-mediated Ca²⁺ mobilization. The results of this theoretical analysis of the effects of GLP-1 on membrane excitability/Ca²⁺ dynamics are discussed in this review.<br>

Journal

• YAKUGAKU ZASSHI

  YAKUGAKU ZASSHI 136 (3), 467-471, 2016-03-01

  The Pharmaceutical Society of Japan

Keywords

• glucagon-like peptide-1
• model stimulation
• Ca²⁺ dynamics

Details

• CRID: 1390282681103264000
• NII Article ID: 130005131244
• NII Book ID: AN00284903
• DOI: 10.1248/yakushi.15-00246-2
• ISSN: 13475231
• ISSN: 00316903
• NDL BIB ID: 027152461
• PubMed: 26935088
• Web Site: http://id.ndl.go.jp/bib/027152461
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027152461
• Web Site: https://www.jstage.jst.go.jp/article/yakushi/136/3/136_15-00246-2/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016310974
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed