Cloning of the short-tailed Gyeongju Donggyeong dog <i>via</i> SCNT: conserving phenotypic inheritance

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Author(s)

    • CHOI Yoo Bin CHOI Yoo Bin
    • Department of Theriogenology and Biotechnology, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea, 151–742
    • KIM Geon A KIM Geon A
    • Department of Theriogenology and Biotechnology, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea, 151–742
    • OH Hyun Ju [他] OH Hyun Ju
    • Department of Theriogenology and Biotechnology, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea, 151–742
    • KIM Min Jung
    • Department of Theriogenology and Biotechnology, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea, 151–742
    • JO Young Kwang
    • Department of Theriogenology and Biotechnology, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea, 151–742
    • SETYAWAN Erif Maha Nugraha
    • Department of Theriogenology and Biotechnology, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea, 151–742
    • LEE Seok Hee
    • Department of Theriogenology and Biotechnology, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea, 151–742
    • LEE Byeong Chun
    • Department of Theriogenology and Biotechnology, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea, 151–742

Abstract

Somatic cell nuclear transfer is a useful tool to maintain genetic information of animals. The Gyeongju Donggyeong dog is a breed registered as natural monument in Korea. The unique feature of the Donggyeong dog is its tail, as the Donggyeong dog can be classified as either short tailed or tailless. The aim of this study was to preserve the Donggyeong dog's unique feature by cloning. Fibroblasts were obtained from a short-tailed Donggyeong dog. <i>In vivo</i> matured oocytes were enucleated, microinjected with a donor cell and fused electrically. Reconstructed embryos were transferred to six recipient dogs. One surrogate became pregnant, and one short-tailed Donggyeong dog was delivered. This study demonstrated that the phenotype of the Donggyeong dog could be conserved by somatic cell nuclear transfer.

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 78(2), 329-331, 2016

    JAPANESE SOCIETY OF VETERINARY SCIENCE

Codes

  • NII Article ID (NAID)
    130005131338
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • ISSN
    0916-7250
  • NDL Article ID
    027141635
  • NDL Call No.
    Z18-350
  • Data Source
    NDL  J-STAGE 
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