Dark Field Microscopic Sensitive Detection of Amyloid Fibrils Using Gold Nanoparticles Modified with Antibody

• BU Tong

  Department of Advanced Materials Science, School of Frontier Sciences, The University of Tokyo Bioengineering Laboratory, RIKEN Institute

• ZAKO Tamotsu

  Bioengineering Laboratory, RIKEN Institute

• MAEDA Mizuo

  Department of Advanced Materials Science, School of Frontier Sciences, The University of Tokyo Bioengineering Laboratory, RIKEN Institute

Abstract

Dark field microscopy (DFM) was employed to detect amyloid β (Aβ) fibrils-induced gold nanoparticle (AuNP) aggregation at the single-particle level, with a detection limit of 40 pM fibrils. The sensitivity of this method is higher than that of the current fibril-specific detection method using probe dye, such as thioflavin T, for which sub-μM level of fibrils are necessary. This study further proved the potential application of DFM in the analytical methods based on AuNP aggregation.

Journal

• Analytical Sciences

  Analytical Sciences 32 (3), 307-311, 2016

  The Japan Society for Analytical Chemistry

Keywords

• Dark field microscopy
• gold nanoparticle
• single molecule analysis
• amyloid β fibrils
• sensitive detection

Details

• CRID: 1390001204259149056
• NII Article ID: 130005131631
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.32.307
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 027203996
• PubMed: 26960610
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027203996
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.32.307.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.32.307/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/32/3/32_307/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016313316
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed