Biocatalytic Formation of Gold Nanoparticles Decorated with Functional Proteins inside Recombinant <i>Escherichia coli</i> Cells

• HOSOMOMI Yukiho

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• NIIDE Teppei

  Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University

• WAKABAYASHI Rie

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• GOTO Masahiro

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University Center for Future Chemistry, Kyushu University

• KAMIYA Noriho

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University Center for Future Chemistry, Kyushu University

Bibliographic Information

Other Title

• Biocatalytic Formation of Gold Nanoparticles Decorated with Functional Proteins inside Recombinant Escherichia coli Cells

Abstract

A novel strategy for the preparation of protein-decorated gold nanoparticles (Au NPs) was developed inside Escherichia coli cells, where an artificial oxidoreductase, composed of antibody-binding protein (pG), Bacillus stearothermophilus glycerol dehydrogenase (BsGLD) and a peptide tag with gold-binding affinity (H₆C), was overexpressed in the cytoplasm. In situ formation of Au NPs was promoted by a natural electron-donating cofactor, nicotinamide adenine dinucleotide (NAD), which was regenerated to the reduced form of NADH by the catalytic activity of the fusion protein (pG-BsGLD-H₆C) overexpressed in the cytoplasm of E. coli, with the concomitant addition of exogenous glycerol to the reaction system. The fusion protein was self-immobilized on Au NPs inside the E. coli cells, which was confirmed by SDS-PAGE and western blotting analyses of the resultant Au NPs. Finally, the IgG binding ability of the pG moiety displayed on Au NPs was evaluated by an enzyme-linked immunosorbent assay.

Journal

• Analytical Sciences

  Analytical Sciences 32 (3), 295-300, 2016

  The Japan Society for Analytical Chemistry

Keywords

• Genetic engineering
• gold nanoparticles
• biocatalyst
• biomineralization
• biosynthesis
• <i>Escherichia coli</i>
• immunoassay

Details

• CRID: 1390001204259150336
• NII Article ID: 130005131633
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.32.295
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 027203941
• PubMed: 26960608
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027203941
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.32.295.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.32.295/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/32/3/32_295/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016313314
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed