Velocity Control of Microtubules with High Spatial Resolution on an Au-coated Surface with an SU-8 Thermal Isolation Layer

• Nakahara Tasuku

  Graduate School of Science and Engineering, Yamaguchi University Department of Micro Engineering, Kyoto University

• Shintaku Hirofumi

  Department of Micro Engineering, Kyoto University

• Kotera Hidetoshi

  Department of Micro Engineering, Kyoto University

• Yokokawa Ryuji

  Department of Micro Engineering, Kyoto University

抄録

The velocity of microtubules gliding on a kinesin-coated surface has been modulated by changing the rate of ATP hydrolysis, temperature, and buffer conditions. Conventional methods have not realized velocity control with high spatial resolution. Here, we propose a method to selectively determine the gliding velocity of microtubules with the help of light irradiance on an Au-coated Si surface. A part of the Au layer was thermally isolated by an underneath coating of SU-8 photoresist, which was employed as a thermal insulation layer. In the area without SU-8, the Au layer was deposited directly on the Si substrate. Irradiation of 13.5 W/cm² controlled the velocity with approximately 40 µm of spatial resolution in the SU-8 coated area, resulting in a velocity of 0.80 ± 0.10 µm/s, whereas in the non-coated area the velocity was 0.62 ± 0.05 µm/s.

収録刊行物

• 電気学会論文誌Ｅ（センサ・マイクロマシン部門誌）

  電気学会論文誌Ｅ（センサ・マイクロマシン部門誌） 136 (3), 77-82, 2016

  一般社団法人 電気学会

キーワード

• Motor proteins
• Molecular transport
• Kinesin
• Microtubule

詳細情報

• CRID: 1390282679436706048
• NII論文ID: 130005131951
• NII書誌ID: AN1052634X
• DOI: 10.1541/ieejsmas.136.77
• ISSN: 13475525
• ISSN: 13418939
• NDL書誌ID: 027160834
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027160834
• Web Site: https://www.jstage.jst.go.jp/article/ieejsmas/136/3/136_77/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可