Low doses of the mycotoxin citrinin protect cortical neurons against glutamate-induced excitotoxicity

• Nakajima Yui

  Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Japan

• Iguchi Hirotoshi

  Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Japan

• Kamisuki Shinji

  Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Japan

• Sugawara Fumio

  Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Japan

• Furuichi Teiichi

  Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Japan

• Shinoda Yo

  Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Japan

Abstract

Citrinin, a natural mycotoxin that is found in fermented foods, is known as a cytotoxin and nephrotoxin. Exposure to high doses of citrinin result in apoptosis; however, the effects of low doses are not fully understood. Glutamate excitotoxicity is responsible for neuronal death in acute neurological disorders including stroke, trauma and other neurodegenerative diseases. Here, we show the neuroprotective effect of low doses of citrinin against glutamate-induced excitotoxicity. We examined the effect of citrinin exposure on glutamate-induced cell death in cultured rat cortical neurons under two conditions: simultaneous treatment with citrinin 0.1 to 1,000 nM and glutamate (30 μM) for 1, 3 hr; the same simultaneous treatment for 3 hr after pretreatment with citrinin for 21 hr. Both the MTT and immunocytochemical assay showed significant neuroprotective effects at several doses and exposure times tested. All concentrations of citrinin tested showed no remarkable cell death following 14-day exposure, and no marked alterations to synapses. These data suggest that low doses of citrinin can be used as a neuroprotective agent against glutamate-induced excitotoxicity without additional harmful cellular alterations.

Journal

• The Journal of Toxicological Sciences

  The Journal of Toxicological Sciences 41 (2), 311-319, 2016

  The Japanese Society of Toxicology

Keywords

• Citrinin
• Excitotoxicity
• Glutamate
• Cortical neuron

Details

• CRID: 1390001204906477056
• NII Article ID: 130005132670
• NII Book ID: AN00002808
• DOI: 10.2131/jts.41.311
• ISSN: 18803989
• ISSN: 03881350
• NDL BIB ID: 027267917
• PubMed: 26961616
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027267917
• Web Site: https://www.jstage.jst.go.jp/article/jts/41/2/41_311/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016296497
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed