Lactic acid bacteria activating innate immunity improve survival in bacterial infection model of silkworm
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- Nishida Satoshi
- Genome Pharmaceutical Institute Co. Ltd. Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo Department of Microbiology and Immunology, Teikyo University School of Medicine
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- Ono Yasuo
- Department of Microbiology and Immunology, Teikyo University School of Medicine
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- Sekimizu Kazuhisa
- Genome Pharmaceutical Institute Co. Ltd. Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
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抄録
Lactic acid bacteria (LAB) have been thought to be helpful for human heath in the gut as probiotics. It recently was noted that activity of LAB stimulating immune systems is important. Innate immune systems are conserved in mammals and insects. Silkworm has innate immunity in response to microbes. Microbe-associated molecular pattern (ex. peptidoglycan and β-glucan) induces a muscle contraction of silkworm larva. In this study, we established an efficient method to isolate lactic acid bacteria derived from natural products. We selected a highly active LAB to activate the innate immunity in silkworm by using the silkworm muscle contraction assay, as well. The assay revealed that Lactococcus lactis 11/19-B1 was highly active on the stimulation of the innate immunity in silkworm. L. lactis 11/19-B1 solely fermented milk with casamino acid and glucose. This strain would be a starter strain to make yogurt. Compared to commercially available yogurt LAB, L. lactis 11/19-B1 has higher activity on silkworm contraction. Silkworm normally ingested an artificial diet mixed with L. lactis 11/19-B1 or a yogurt fermented with L. lactis 11/19-B1. Interestingly, silkworms that ingested the LAB showed tolerance against the pathogenicity of Pseudomonas aeruginosa. These data suggest that Lactococcus lactis 11/19-B1 would be expected to be useful for making yogurt and probiotics to activate innate immunity.
収録刊行物
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- Drug Discoveries & Therapeutics
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Drug Discoveries & Therapeutics 10 (1), 49-56, 2016
特定非営利活動法人 バイオ＆ソーシャル・サイエンス推進国際研究交流会