MALDI In-Source Decay of Protein

• Takayama Mitsuo

  Mass Spectrometry Laboratory, Graduate School of Nanobioscience, Yokohama City University

Bibliographic Information

Other Title

• MALDI in-source decay of protein: the mechanism of c-ion formation
• The Mechanism of <i>c</i>-Ion Formation

Abstract

The in-source decay (ISD) phenomenon, the fragmentation at an N–C_α bond of a peptide backbone that occurs within several tens of nanoseconds in the ion-source in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS), is discussed from the standpoints of the discovery and early publications dealing with MALDI-ISD, the formation of c-ions in energy-sudden desorption/ionization methods, the formation of radical species in a MALDI, model construction for ISD, and matrix materials that are suitable for use in MALDI-ISD. The formation of c-ions derived from peptides and proteins in MALDI-ISD can be rationalized by a mechanism involving intermolecular hydrogen transfer, denoted as the “Takayama’s model” by De Pauw’s group (Anal. Chem. 79: 8678–8685, 2007). It should be emphasized that the model for MALDI-ISD was constructed on the basis of X-ray crystallography and scanning probe microscopy (SPM) analyses of matrix crystals, as well as the use of isotopically-labelled peptides.

Journal

• Mass Spectrometry

  Mass Spectrometry 5 (1), A0044-A0044, 2016

  The Mass Spectrometry Society of Japan

Keywords

• MALDI
• in-source decay
• mechanism
• <i>c</i>-ion

Details

• CRID: 1390001205415028992
• NII Article ID: 130005138385
• DOI: 10.5702/massspectrometry.a0044
• ISSN: 21865116
• ISSN: 2187137X
• Web Site: https://www.jstage.jst.go.jp/article/massspectrometry/5/1/5_A0044/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed