Direct Detection of Fe(Ⅱ) in Extracellular Polymeric Substances (EPS) at the Mineral-Microbe Interface in Bacterial Pyrite Leaching Direct Detection of Fe(II) in Extracellular Polymeric Substances (EPS) at the Mineral-Microbe Interface in Bacterial Pyrite Leaching

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Author(s)

    • ZHU MING Mase Kazuhiko
    • Institute of Materials Structure Science, High-Energy Accelerator Research Organization (KEK)|The Graduate University for Advanced Studies
    • Zhu Ming
    • Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka
    • Takeichi Yasuo
    • Institute of Materials Structure Science, High-Energy Accelerator Research Organization (KEK)|The Graduate University for Advanced Studies
    • Suga Hiroki
    • Department of Earth and Planetary Systems Science, Hiroshima University
    • Jinno Muneaki
    • Institute of Materials Structure Science, High-Energy Accelerator Research Organization (KEK)|Toyama Co. Ltd.
    • Makita Hiroko
    • Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
    • Sakata Masahiro
    • Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka
    • Ono Kanta
    • Institute of Materials Structure Science, High-Energy Accelerator Research Organization (KEK)|The Graduate University for Advanced Studies

Abstract

We herein investigated the mechanisms underlying the contact leaching process in pyrite bioleaching by <i>Acidithiobacillus ferrooxidans</i> using scanning transmission X-ray microscopy (STXM)-based C and Fe near edge X-ray absorption fine structure (NEXAFS) analyses. The C NEXAFS analysis directly showed that attached <i>A. ferrooxidans</i> produces polysaccharide-abundant extracellular polymeric substances (EPS) at the cell-pyrite interface. Furthermore, by combining the C and Fe NEXAFS results, we detected significant amounts of Fe(II), in addition to Fe(III), in the interfacial EPS at the cell-pyrite interface. A probable explanation for the Fe(II) in detected EPS is the leaching of Fe(II) from the pyrite. The detection of Fe(II) also indicates that Fe(III) resulting from pyrite oxidation may effectively function as an oxidizing agent for pyrite at the cell-pyrite interface. Thus, our results imply that a key role of Fe(III) in EPS, in addition to its previously described role in the electrostatic attachment of the cell to pyrite, is enhancing pyrite dissolution.

Journal

  • Microbes and Environments

    Microbes and Environments 31(1), 63-69, 2016

    Japanese Society of Microbial Ecology · The Japanese Society of Soil Microbiology

Codes

  • NII Article ID (NAID)
    130005138797
  • NII NACSIS-CAT ID (NCID)
    AA11551577
  • Text Lang
    ENG
  • ISSN
    1342-6311
  • NDL Article ID
    027200800
  • NDL Call No.
    Z54-J644
  • Data Source
    NDL  J-STAGE 
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