電気化学免疫測定法(ECLIA法)を用いたtacrolimusおよびcyclosporin A血中濃度測定に関する臨床的評価
書誌事項
- タイトル別名
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- Assessment of electro chemiluminescence immunoassay method for measuring tacrolimus and cyclosporine A blood concentrations in clinical practice
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【Objective】Therapeutic drug monitoring (TDM) of tacrolimus and cyclosporine A is essential to maintain the safety and efficacy in personalized immunosuppressive therapy. This study was to evaluate the recently developed electro chemiluminescence immunoassay (ECLIA) method for measuring tacrolimus and cyclosporine A blood concentrations in clinical practice.<br/>【Methods】Spiked whole-blood samples were used to evaluate the performance of ECLIA. Whole-blood tacrolimus (n=160) or cyclosporine A (n=140) concentrations of transplanted patients were measured by ECLIA, chemiluminescence immunoassay (CLIA), affinity column mediated immune assay (ACMIA), and liquid chromatography with tandem mass spectrometry (LC-MS/MS).<br/>【Results】The ECLIA measurements of tacrolimus and cyclosporine A showed good performance in terms of sensitivity, repeatability, and specificity. Values obtained with the ECLIA were highly correlated with the CLIA for tacrolimus (ECLIA= 1.013×CLIA - 0.055; r=0.996), with the LC-MS/MS for tacrolimus (ECLIA=0.945×LC-MS/MS + 0.630; r=0.983), with the ACMIA for cyclosporine A (ECLIA = 1.099×ACMIA - 18.999; r= 0.989) and with the LC-MS/MS for cyclosporine A (ECLIA = 0.890×LC-MS/MS + 27.099; r= 0.977).<br/>【Conclusion】The ECLIA is a sensitive and useful method to routinely monitor tacrolimus and cyclosporine A concentration in whole blood.
収録刊行物
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- 移植
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移植 51 (1), 058-065, 2016
一般社団法人 日本移植学会
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詳細情報 詳細情報について
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- CRID
- 1390001205511304448
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- NII論文ID
- 130005139942
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- ISSN
- 21880034
- 05787947
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可