Real-time PCR genotyping assay for canine progressive rod-cone degeneration and mutant allele frequency in Toy Poodles, Chihuahuas and Miniature Dachshunds in Japan

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Author(s)

    • KOHYAMA Moeko KOHYAMA Moeko
    • Laboratory of Clinical Pathology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1–21–24 Kohrimoto, Kagoshima 890–0065, Japan
    • TADA Naomi YAMATO Osamu
    • Laboratory of Clinical Pathology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1–21–24 Kohrimoto, Kagoshima 890–0065, Japan
    • MITSUI Hiroko TADA Naomi
    • International Institute of Small Animal Medicine (Bio Plus), AHB Inc., 3–7–11 Kiba, Koutou-ku, Tokyo 135–0042, Japan
    • TOMIOKA Hitomi MITSUI Hiroko
    • International Institute of Small Animal Medicine (Bio Plus), AHB Inc., 3–7–11 Kiba, Koutou-ku, Tokyo 135–0042, Japan
    • MIZUKAMI Keijiro KUSHIDA Kazuya
    • Laboratory of Clinical Pathology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1–21–24 Kohrimoto, Kagoshima 890–0065, Japan
    • YAMATO Osamu MIZUKAMI Keijiro
    • Laboratory of Clinical Pathology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1–21–24 Kohrimoto, Kagoshima 890–0065, Japan

Abstract

Canine progressive rod-cone degeneration (PRCD) is a middle- to late-onset, autosomal recessive, inherited retinal disorder caused by a substitution (c.5G>A) in the canine <i>PRCD</i> gene that has been identified in 29 or more purebred dogs. In the present study, a TaqMan probe-based real-time PCR assay was developed and evaluated for rapid genotyping and large-scale screening of the mutation. Furthermore, a genotyping survey was carried out in a population of the three most popular breeds in Japan (Toy Poodles, Chihuahuas and Miniature Dachshunds) to determine the current mutant allele frequency. The assay separated all the genotypes of canine PRCD rapidly, indicating its suitability for large-scale surveys. The results of the survey showed that the mutant allele frequency in Toy Poodles was high enough (approximately 0.09) to allow the establishment of measures for the prevention and control of this disorder in breeding kennels. The mutant allele was detected in Chihuahuas for the first time, but the frequency was lower (approximately 0.02) than that in Toy Poodles. The mutant allele was not detected in Miniature Dachshunds. This assay will allow the selective breeding of dogs from the two most popular breeds (Toy Poodle and Chihuahua) in Japan and effective prevention or control of the disorder.

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 78(3), 481-484, 2016

    JAPANESE SOCIETY OF VETERINARY SCIENCE

Codes

  • NII Article ID (NAID)
    130005140895
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • ISSN
    0916-7250
  • NDL Article ID
    027226929
  • NDL Call No.
    Z18-350
  • Data Source
    NDL  J-STAGE 
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