The epidemiological survey for atovaquone resistant related gene of <i>Babesia gibsoni</i> in Japan
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In 73 gDNA samples from <i>Babesia gibsoni-</i>infected dogs, the M121I variant population was measured by using allele-specific real-time PCR. Although the mechanism of atovaquone against <i>B. gibsoni</i> has not been clearly identified, it is reported that the mitochondria cytochrome <i>b</i> gene of the atovaquone-resistant <i>B. gibsoni</i> had a single-nucleotide substitution at nt363 (G to T), which resulted in the substitution of methionine with isoleucine (M121I). In this study, 3/73 samples showed over 5% M121I variant population. Although the M121I variant population is a low percentage, it runs the risk of spreading drug-resistant parasites. It is important to prevent the spread of drug-resistance, so we need to gather information about this at regular intervals.
- Journal of Veterinary Medical Science
Journal of Veterinary Medical Science 78(3), 489-491, 2016
Japanese Society of Veterinary Science