The epidemiological survey for atovaquone resistant related gene of <i>Babesia gibsoni</i> in Japan

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Author(s)

    • IGUCHI Aiko IGUCHI Aiko
    • National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080–8555, Japan
    • SUZUKI Hiroshi SUZUKI Hiroshi
    • National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080–8555, Japan
    • XUAN Xuenan XUAN Xuenan
    • National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080–8555, Japan

Abstract

In 73 gDNA samples from <i>Babesia gibsoni-</i>infected dogs, the M121I variant population was measured by using allele-specific real-time PCR. Although the mechanism of atovaquone against <i>B. gibsoni</i> has not been clearly identified, it is reported that the mitochondria cytochrome <i>b</i> gene of the atovaquone-resistant <i>B. gibsoni</i> had a single-nucleotide substitution at nt363 (G to T), which resulted in the substitution of methionine with isoleucine (M121I). In this study, 3/73 samples showed over 5% M121I variant population. Although the M121I variant population is a low percentage, it runs the risk of spreading drug-resistant parasites. It is important to prevent the spread of drug-resistance, so we need to gather information about this at regular intervals.

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 78(3), 489-491, 2016

    Japanese Society of Veterinary Science

Codes

  • NII Article ID (NAID)
    130005140900
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    0916-7250
  • NDL Article ID
    027226947
  • NDL Call No.
    Z18-350
  • Data Source
    NDL  IR  J-STAGE 
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