Direct evidence of the glucose uptake into cochlear strial marginal cells: Application of a fluorescent tracer method combined with immunohistochemistry

DOI
  • Hishikawa Sohta
    Laboratory of Cell Physiology, Department of Science Education, Graduate School of Education, Okayama University
  • Edamatsu Midori
    Department of Molecular Biology and Biochemistry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • Inoue-Ikeda Risa
    Laboratory of Animal Physiology and Pharmacology, Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
  • Ando Motonori
    Laboratory of Cell Physiology, Department of Science Education, Graduate School of Education, Okayama University Laboratory of Animal Physiology and Pharmacology, Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University

この論文をさがす

抄録

Despite the importance of glucose as an energy source for marginal cells of the cochlear stria vascularis, no direct evidence of the glucose uptake into these cells has been demonstrated. In this study, we investigated the glucose pathway in strial marginal cells using a fluorescent tracer method combined with immunohistochemistry. For glucose imaging, a non-metabolizable fluorescent glucose analog, 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-6-deoxyglucose (6-NBDG), was intravenously injected into mice. After 6-NBDG fluorescent signals in cochlear sections were observed, the same sections were processed for immunohistochemistry to identify marginal cells. This showed that 6-NBDG fluorescent signals had passed into the cytosol of marginal cells via Na+/K+-ATPase-positive basolateral membranes. These results provide direct physiological evidence of the glucose uptake into epithelial strial marginal cells. This combined method can help in examining the relationship between target molecules and functional proteins using the same tissue samples.

収録刊行物

  • bioimages

    bioimages 23 (0), 1-8, 2015

    日本バイオイメージング学会

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ