N, N-Dimethylglycine decreases oxidative stress and improves <i>in vitro</i> development of bovine embryos

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Author(s)

Abstract

The antioxidant effect of N, N-dimethylglycine (DMG) on <i>in vitro</i>-produced (IVP) bovine embryos was examined. After <i>in vitro</i> fertilization, presumptive zygotes were cultured with or without 0.1 μM DMG under different oxygen tensions. The percentage of embryos developing to the blastocyst stage was lowest under a 20% oxygen concentration without DMG, and it was significantly increased (P < 0.05) by applying a 5% oxygen concentration. Under the 20% oxygen concentration, supplementation of the medium with DMG significantly improved blastocyst development, which was nearly equal to that achieved under 5% oxygen without DMG. Furthermore, a tendentious increase (P = 0.06) in blastocyst cell numbers was observed when DMG was applied. In the second experiment, addition of H<sub>2</sub>O<sub>2</sub> (0.5 mM) to the culture medium significantly (P < 0.01) reduced the percentage of embryos developing to the blastocyst stage. However, DMG supplementation prevented this reduction. In conclusion, DMG enhanced the <i>in</i><i>vitro</i> development of IVP bovine embryos by acting as an antioxidant.

Journal

  • Journal of Reproduction and Development

    Journal of Reproduction and Development 62(2), 209-212, 2016

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

Codes

  • NII Article ID (NAID)
    130005146469
  • NII NACSIS-CAT ID (NCID)
    AA10936678
  • Text Lang
    ENG
  • ISSN
    0916-8818
  • NDL Article ID
    027270002
  • NDL Call No.
    Z54-H305
  • Data Source
    NDL  J-STAGE 
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