N, N-Dimethylglycine decreases oxidative stress and improves <i>in vitro</i> development of bovine embryos
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The antioxidant effect of N, N-dimethylglycine (DMG) on <i>in vitro</i>-produced (IVP) bovine embryos was examined. After <i>in vitro</i> fertilization, presumptive zygotes were cultured with or without 0.1 μM DMG under different oxygen tensions. The percentage of embryos developing to the blastocyst stage was lowest under a 20% oxygen concentration without DMG, and it was significantly increased (P < 0.05) by applying a 5% oxygen concentration. Under the 20% oxygen concentration, supplementation of the medium with DMG significantly improved blastocyst development, which was nearly equal to that achieved under 5% oxygen without DMG. Furthermore, a tendentious increase (P = 0.06) in blastocyst cell numbers was observed when DMG was applied. In the second experiment, addition of H<sub>2</sub>O<sub>2</sub> (0.5 mM) to the culture medium significantly (P < 0.01) reduced the percentage of embryos developing to the blastocyst stage. However, DMG supplementation prevented this reduction. In conclusion, DMG enhanced the <i>in</i><i>vitro</i> development of IVP bovine embryos by acting as an antioxidant.
- Journal of Reproduction and Development
Journal of Reproduction and Development 62(2), 209-212, 2016
THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT