Capric Acid Up-Regulates UCP3 Expression without PDK4 Induction in Mouse C2C12 Myotubes

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Author(s)

    • HIRASAKA Katsuya OKUMURA Yuushi
    • Departments of Nutritional Physiology, Tokushima University Graduate School|Graduate School of Nutritional Sciences, Sagami Women’s University
    • TERAO Junji
    • Food Science, Institute of Biomedical Sciences, Tokushima University Graduate School
    • NIKAWA Takeshi
    • Departments of Nutritional Physiology, Tokushima University Graduate School
    • HIRASAKA Katsuya
    • Departments of Nutritional Physiology, Tokushima University Graduate School|Graduate School of Fisheries Science and Environmental Studies, Nagasaki University
    • KOHNO Shohei
    • Departments of Nutritional Physiology, Tokushima University Graduate School|Pharmacology/Toxicology, College of Pharmacy, University of Texas
    • TOMIDA Chisato
    • Departments of Nutritional Physiology, Tokushima University Graduate School
    • HARUNA Marie
    • Departments of Nutritional Physiology, Tokushima University Graduate School
    • UCHIDA Takayuki
    • Departments of Nutritional Physiology, Tokushima University Graduate School
    • OHNO Ayako
    • Departments of Nutritional Physiology, Tokushima University Graduate School

Abstract

Uncoupling protein 3 (UCP3) and pyruvate dehydrogenase kinase 4 (PDK4) in skeletal muscle are key regulators of the glucose and lipid metabolic processes that are involved in insulin resistance. Medium-chain fatty acids (MCFAs) have anti-obesogenic effects in rodents and humans, while long-chain fatty acids (LCFAs) cause increases in body weight and insulin resistance. To clarify the beneficial effects of MCFAs, we examined <i>UCP3</i> and <i>PDK4</i> expression in skeletal muscles of mice fed a MCFA- or LCFA-enriched high-fat diet (HFD). Five-week feeding of the LCFA-enriched HFD caused high body weight gain and induced glucose intolerance in mice, compared with those in mice fed the MCFA-enriched HFD. However, the amounts of <i>UCP3</i> and <i>PDK4</i> transcripts in the skeletal muscle of mice fed the MCFA- or LCFA-enriched HFD were similar. To further elucidate the specific effects of MCFAs, such as capric acid (C10:0), on lipid metabolism in skeletal muscles, we examined the effects of various FAs on expression of <i>UCP3</i> and <i>PDK4</i>, in mouse C2C12 myocytes. Although palmitic acid (C16:0) and lauric acid (C12:0) significantly induced expression of both <i>UCP3</i> and <i>PDK4</i>, capric acid (C10:0) upregulated only <i>UCP3</i> expression via activation of peroxisome proliferator-activated receptor-δ. Furthermore, palmitic acid (C16:0) disturbed the insulin-induced phosphorylation of Akt, while MCFAs, including lauric (C12:0), capric (C10:0), and caprylic acid (C12:0), did not. These results suggest that capric acid (C10:0) increases the capacity for fatty acid oxidation without inhibiting glycolysis in skeletal muscle.

Journal

  • Journal of Nutritional Science and Vitaminology

    Journal of Nutritional Science and Vitaminology 62(1), 32-39, 2016

    Center for Academic Publications Japan

Codes

  • NII Article ID (NAID)
    130005148603
  • NII NACSIS-CAT ID (NCID)
    AA00703822
  • Text Lang
    ENG
  • ISSN
    0301-4800
  • NDL Article ID
    027140218
  • NDL Call No.
    Z53-B484
  • Data Source
    NDL  J-STAGE 
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