象牙質の成長線の周期と体内時計の情報伝達分子のメラトニンの分泌リズムの関連  [in Japanese] The Connection between the Periodicity Incremental Lines of Tooth Dentin and the Secretion Rhythm of Melatonin of the Signaling Molecules of Biological Clock  [in Japanese]

Access this Article

Search this Article

Author(s)

    • 門田 理佳 KADOTA Rika
    • 高知学園短期大学専攻科応用生命科学専攻, Applied Life Science Course, Kochi Gakuen College, Kochi, Japan
    • 服部 淳彦 HATTORI Atsuhiko
    • 東京医科歯科大学教養部生物学, Department of Biology, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Ichikawa, Japan
    • 鈴木 信雄 SUZUKI Nobuo
    • 金沢大学環日本海域環境研究センター, Institute of Nature and Environmental Technology, Kanazawa University, Housu-gun, Japan
    • 筧 光夫 KAKEI Mitsuo
    • 明海大学歯学部口腔解剖学分野, Division of Oral Anatomy, Meikai University School of Dentistry, Sakado, Japan
    • 松本 敬 MATUMOTO Takashi
    • 日本大学松戸歯学部付属病院病理診断科 Deptartment of Laboratory Diagnostic, University Hospital, Nihon University School of Dentistry at Matsudo, Matsudo, Japan
    • 里村 一人 SATOMURA Kazuto
    • 鶴見大学歯学部口腔内科学講座, Department of Oral Medicine and Stomatology, School of Dental Medicine, Turumi University, Yokohama, Japan
    • 見明 康雄 MIAKE Yasuo
    • 東京歯科大学口腔超微構造学講座 Depertment of Ultrastructural Science, Oral Health Science Center, Tokyo Dental College, Tokyo, Japan

Abstract

本研究はメラトニンと象牙質における成長線の周期性との関連を解明し,その作用機序を探ることを目的とした.対照群とメラトニン投与の低濃度群及び高濃度群の3群に分けて実験した.研究は①5日令の歯根形成期の夜間と次の日の6日令の昼間及び②7日令の昼間と次の日の8日令の夜間の試料を用いた.低濃度群では濃染層の幅が広がり,淡染層の幅が狭くなっていた.高濃度群では成長線の間の淡染層は認められなかった. 低濃度群や高濃度群では象牙前質に石灰化球が数多く観察された.メラトニン投与濃度が上昇するにつれて象牙前質中の石灰化球の数と大きさは有意に増加していた.メラトニン投与群では,切歯と臼歯の歯冠象牙質の中央部に新しい成長線が観察された.SEM-EDS分析では,CaとP含有量がメラトニン投与群で増加していた.メラトニンが象牙芽細胞の石灰化周期とコラーゲン分泌周期を促進し,成長線の形成機構に影響されたと考察される.

The purpose of the present study is to examine the relationship between the periodicity of incremental lines in dentin of teeth and the role of melatonin. In this experiment, 5- and 6-day, 7- and 8-days after birth SD rats were used. In the low melatonin concentration group, the light staining layer became narrow. In the high melatonin concentration group, this layer disappeared. The number and size of calcospherites in predentin increased in proportion to the concentration of melatonin administered. The new incremental line was confirmed in both the incisor and molar dentin of the melatonin treated groups. In SEM-EDS analysis, Ca and P content were increasing by the melatonin treated group compared with the control group. It is considered that melatonin participates in the formation of incremental lines and the calcification mechanism of the odontoblast. The work was supported by KAKENHI (23592727).

Journal

  • Journal of the Japanese Association of Regenerative Dentistry

    Journal of the Japanese Association of Regenerative Dentistry 11(1), 27-39, 2013

    Japanese Association of Regenerative Dentistry

Codes

  • NII Article ID (NAID)
    130005150558
  • Text Lang
    JPN
  • ISSN
    1348-9615
  • Data Source
    J-STAGE 
Page Top