Direct Imaging Mass Spectrometry of Plant Leaves Using Surface-assisted Laser Desorption/Ionization with Sputter-deposited Platinum Film

• OZAWA Tomoyuki

  Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials, and Bioengineering, Kansai University

• OSAKA Issey

  Center for Nano Materials and Technology, Japan Advanced Institute of Science and Technology

• HAMADA Satoshi

  Analysis Research Department, Chemical Research Laboratories, Nissan Chemical Industries, Ltd.

• MURAKAMI Tatsuya

  Center for Nano Materials and Technology, Japan Advanced Institute of Science and Technology

• MIYAZATO Akio

  Center for Nano Materials and Technology, Japan Advanced Institute of Science and Technology

• KAWASAKI Hideya

  Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials, and Bioengineering, Kansai University

• ARAKAWA Ryuichi

  Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials, and Bioengineering, Kansai University

抄録

Plant leaves administered with systemic insecticides as agricultural chemicals were analyzed using imaging mass spectrometry (IMS). Matrix-assisted laser desorption/ionization (MALDI) is inadequate for the detection of insecticides on leaves because of the charge-up effect that occurs on the non-conductive surface of the leaves. In this study, surface-assisted laser desorption/ionization with a sputter-deposited platinum film (Pt-SALDI) was used for direct analysis of chemicals in plant leaves. Sputter-deposited platinum (Pt) films were prepared on leaves administered with the insecticides. A sputter-deposited Pt film with porous structure was used as the matrix for Pt-SALDI. Acephate and acetamiprid contained in the insecticides on the leaves could be detected using Pt-SALDI-MS, but these chemical components could not be adequately detected using MALDI-MS because of the charge-up effect. Enhancement of ion yields for the insecticides was achieved using Pt-SALDI, accompanied by prevention of the charge-up effect by the conductive Pt film. The movement of systemic insecticides in plants could be observed clearly using Pt-SALDI-IMS. The distribution and movement of components of systemic insecticides on leaves could be analyzed directly using Pt-SALDI-IMS. Additionally, changes in the properties of the chemicals with time, as an indicator of the permeability of the insecticides, could be evaluated.

収録刊行物

• Analytical Sciences

  Analytical Sciences 32 (5), 587-591, 2016

  社団法人 日本分析化学会

キーワード

• Pt film
• imaging mass spectrometry (IMS)
• surface-assisted laser desorption ionization (SALDI)
• leaf
• agricultural chemicals

詳細情報

• CRID: 1390001204260158848
• NII論文ID: 130005150936
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.32.587
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 027283880
• PubMed: 27169661
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027283880
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.32.587.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.32.587/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/32/5/32_587/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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