Transient effects of empty liposomes on hepatic macrophage populations in rats

• Pervin Munmun

  Laboratory of Veterinary Pathology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University,1-58 Rinku-ourai-kita, Izumisano City, Osaka 598-8531, Japan

• Golbar Hossain M.

  Laboratory of Veterinary Pathology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University,1-58 Rinku-ourai-kita, Izumisano City, Osaka 598-8531, Japan

• Bondoc Alexandra

  Laboratory of Veterinary Pathology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University,1-58 Rinku-ourai-kita, Izumisano City, Osaka 598-8531, Japan

• Izawa Takeshi

  Laboratory of Veterinary Pathology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University,1-58 Rinku-ourai-kita, Izumisano City, Osaka 598-8531, Japan

• Kuwamura Mitsuru

  Laboratory of Veterinary Pathology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University,1-58 Rinku-ourai-kita, Izumisano City, Osaka 598-8531, Japan

• Yamate Jyoji

  Laboratory of Veterinary Pathology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University,1-58 Rinku-ourai-kita, Izumisano City, Osaka 598-8531, Japan

Abstract

Liposomes have been used as a vehicle for encapsulating chemicals or toxins in toxicological studies. We investigated the transient effects of empty liposomes on hepatic macrophages by applying a single intravenous injection at a dose of 10 ml/kg body weight in 6-week-old male F344 rats. One day after injection, the numbers of hepatic macrophages reacting to CD163, CD68, Iba-1, MHC class II, Gal-3 and CD204 were significantly increased in liposome-treated rats. CD163⁺ Kupffer cells and CD68⁺ macrophages with increased phagocytic activity in hepatic lobules were most sensitive. The histological architecture of the liver was not changed following liposome injection; however, hepatocytes showed increased proliferating activity, demonstrable with proliferation marker immunostaining and by an increase in gene profiles related to the cell cycle. In the liposome-treated rats, interestingly, AST and ALT values were significantly decreased, and MCP-1, IL-1β and TGF-β1 mRNAs were significantly increased. Collectively, the present study found that hepatic macrophages activated by liposomes can influence liver homeostasis. This information would be useful for background studies on liposomes.

Journal

• Journal of Toxicologic Pathology

  Journal of Toxicologic Pathology 29 (2), 139-144, 2016

  JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY

Keywords

• liposome
• hepatic macrophages
• immunohistochemistry
• homeostasis
• rats

Details

• CRID: 1390001204416378752
• NII Article ID: 130005151011
• NII Book ID: AN10232280
• DOI: 10.1293/tox.2015-0082
• ISSN: 1881915X
• ISSN: 09149198
• NDL BIB ID: 027347427
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027347427
• Web Site: https://www.jstage.jst.go.jp/article/tox/29/2/29_2015-0082/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016381769
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed