Association between p53-binding protein 1 expression and genomic instability in oncocytic follicular adenoma of the thyroid

Access this Article

Author(s)

    • Mussazhanova Zhanna
    • Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan
    • Rogounovitch Tatiana
    • Department of Radiation Medical Sciences, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan
    • Saenko Vladimir
    • Department of Radiation Medical Sciences, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan
    • Kozykenova Zhanna
    • Department of Physiological Disciplines, Semey State Medical University, Semey 071400, Kazakhstan
    • Zhetpisbaev Bekbolat
    • Department of Physiological Disciplines, Semey State Medical University, Semey 071400, Kazakhstan
    • Shabdarbaeva Dariya
    • Department of Pathological Anatomy and Forensic Medicine, Semey State Medical University, Semey 071400, Kazakhstan
    • Sayakenov Nurlan
    • Department of Pathological Anatomy and Forensic Medicine, Semey State Medical University, Semey 071400, Kazakhstan
    • Amantayev Bakanay
    • Department of Operative Dentistry, Asfendiyarov Kazakh National Medical University, Almaty 050000, Kazakhstan
    • Kondo Hisayoshi
    • Biostatics Section, Division of Scientific Data Registry, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan
    • Ito Masahiro
    • Department of Pathology, National Hospital Organization Nagasaki Medical Center, Omura 856-8562, Japan
    • Nakashima Masahiro
    • Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan
    • Akazawa Yuko
    • Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan|Department of Gastroenterology and Hepatology, Nagasaki University Hospital, Nagasaki 852-8501, Japan
    • Matsuda Katsuya
    • Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan
    • Shichijo Kazuko
    • Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan
    • Miura Shiro
    • Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan
    • Otsubo Ryota
    • Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan|Division of Surgical Oncology, Department of Translational Medical Sciences, Nagasaki University, Nagasaki 852-8501, Japan
    • Oikawa Masahiro
    • Division of Surgical Oncology, Department of Translational Medical Sciences, Nagasaki University, Nagasaki 852-8501, Japan|Department of Human Genetics, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan
    • Yoshiura Koh-ichiro
    • Department of Human Genetics, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan
    • Mitsutake Norisato
    • Department of Radiation Medical Sciences, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan

Abstract

Oncocytic follicular adenomas (FAs) of the thyroid are neoplasms of follicular cell origin that are predominantly composed of large polygonal cells with eosinophilic and granular cytoplasm. However, the pathological characteristics of these tumors are largely unexplored. Both the initiation and progression of cancer can be caused by an accumulation of genetic mutations that can induce genomic instability. Thus, the aim of this study was to evaluate the extent of genomic instability in oncocytic FA. As the presence of p53-binding protein 1 (53BP1) in nuclear foci has been found to reflect DNA double-strand breaks that are triggered by various stresses, the immunofluorescence expression pattern of 53BP-1 was assessed in oncocytic and conventional FA. The association with the degree of DNA copy number aberration (CNA) was also evaluated using array-based comparative genomic hybridization. Data from this study demonstrated increased 53BP1 expression (<i>i.e.</i>, “unstable” expression) in nuclear foci of oncocytic FA and a higher incidence of CNAs compared with conventional FA. There was also a particular focus on the amplification of chromosome 1p36 in oncocytic FA, which includes the locus for Tumor protein 73, a member of the p53 family implicated as a factor in the development of malignancies. Further evaluations revealed that unstable 53BP1 expression had a significant positive correlation with the levels of expression of Tumor protein 73. These data suggest a higher level of genomic instability in oncocytic FA compared with conventional FA, and a possible relationship between oncocytic FA and abnormal amplification of Tumor protein 73.

Journal

  • Endocrine Journal

    Endocrine Journal 63(5), 457-467, 2016

    The Japan Endocrine Society

Codes

Page Top