Detection of Capsaicinoids in Capsicum Fruit Using Surface Plasmon Resonance Immunosensor

• Onodera Takeshi

  Research and Development Center for Taste and Odor Sensing, Kyushu University

• Tian Shengxi

  Graduate School and Faculty of information Science and Electrical Engineering, Kyushu University

• Yatabe Rui

  Research and Development Center for Taste and Odor Sensing, Kyushu University

• Toko Kiyoshi

  Research and Development Center for Taste and Odor Sensing, Kyushu University Graduate School and Faculty of information Science and Electrical Engineering, Kyushu University

Bibliographic Information

Other Title

• 表面プラズモン共鳴免疫センサを用いたトウガラシ果実中のカプサイシノイド検出
• ヒョウメン プラズモン キョウメイ メンエキ センサ オ モチイタ トウガラシ カジツ チュウ ノ カプサイシノイド ケンシュツ

Abstract

Detection method of capsaicinoids such as capsaicin, dihydrocapsaicin and nordihydrocapsaicin was developed using anti-homovalic acid-Concholepas concholepas hemocyanin (CCH) polyclonal antibodies and surface plasmon resonance (SPR) sensor with indirect competitive assay method. Extraction procedure of capsaicinoids from Capsicum fruit was also developed. As a result, limits of detection for capsaicin, dihidrocapsaicin and nordihydrocapsaicin were 27 ppb, 35 ppb and 50 ppb, respectively. The reduction of responses in SPR measurements with indirect competitive method for recovered samples of sweet peppers such as Bell pepper and Shishitougarashi were not observed. Nine kinds of hot peppers (eg. Habanero, Trinidad scorpion), which have different Scovile heat unit (SHU), were also tested. The reductions of sensor responses for the hot peppers were observed. It is likely that samples extracted from Capsicum fruit of the hot peppers contained capsaicinoids. It was found that the responses of the SPR immunosensor depended on SHU of each hot pepper.

Journal

• IEEJ Transactions on Sensors and Micromachines

  IEEJ Transactions on Sensors and Micromachines 136 (6), 218-223, 2016

  The Institute of Electrical Engineers of Japan

Keywords

• capsaicin
• capsaicinoid
• surface plasmon resonance
• pungency
• antigen-antibody interaction
• immunosensor

Details

• CRID: 1390001204462513792
• NII Article ID: 130005154783
• NII Book ID: AN1052634X
• DOI: 10.1541/ieejsmas.136.218
• ISSN: 13475525
• ISSN: 13418939
• NDL BIB ID: 027488622
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027488622
• Web Site: https://www.jstage.jst.go.jp/article/ieejsmas/136/6/136_218/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed