ボスカリド耐性ナスすすかび病菌の発生とPCR-RFLPによる検出  [in Japanese] Occurrence of boscalid-resistant <i>Mycovellosiella nattrassii</i> isolates from eggplant and detection using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).  [in Japanese]

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Abstract

2011年に高知県ナス圃場から単胞子分離されたすすかび病菌のボスカリドに対する感受性を薬剤添加培地にて調べたところ,EC<sub>50</sub>が16を超える菌株が25菌株中6菌株検出され,接種検定により耐性菌であることを確認した.それらは<i>SdhB</i>遺伝子に共通の遺伝子変異を持ち,この遺伝子変異はPCR-RFLP法で検出可能であった.ボスカリドと同じSDHI剤であるペンチオピラドは今回発生したボスカリド耐性菌に対し,生物検定で防除効果が認められ,交叉性を示さなかった.

In 2011, 25 isolates of <i>Mycovellosiella nattrassii</i> causing leaf mold on eggplant were collected from eggplant fields in Kochi Prefecture and tested for sensitivity to the succinate dehydrogenase inhibitor (SDHI) boscalid. In a test to determine the effective fungicide concentration that inhibits mycelia growth 50% relative to the control (EC<sub>50</sub>), 6 of 25 isolates were less sensitive to boscalid (EC<sub>50</sub> > 16 ppm). They were resistant to boscalid but not to pentiopyrad in an inoculation test. A common nucleotide change was found in their <i>SdhB</i> gene encoding a target protein of boscalid, and the mutation was detected by PCR-RFLP.

Journal

  • Japanese Journal of Phytopathology

    Japanese Journal of Phytopathology 82(2), 87-92, 2016

    The Phytopathological Society of Japan

Codes

  • NII Article ID (NAID)
    130005156359
  • NII NACSIS-CAT ID (NCID)
    AN0019269X
  • Text Lang
    JPN
  • ISSN
    0031-9473
  • NDL Article ID
    027470170
  • NDL Call No.
    Z18-347
  • Data Source
    NDL  J-STAGE 
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