Liver condition of Holstein cows affects mitochondrial function and fertilization ability of oocytes
-
- TANAKA Hiroshi
- Tokyo University of Agriculture, Kanagawa 243-0034, Japan
-
- TAKEO Shun
- Tokyo University of Agriculture, Kanagawa 243-0034, Japan
-
- ABE Takahito
- Tokyo University of Agriculture, Kanagawa 243-0034, Japan
-
- KIN Airi
- Tokyo University of Agriculture, Kanagawa 243-0034, Japan
-
- SHIRASUNA Koumei
- Tokyo University of Agriculture, Kanagawa 243-0034, Japan
-
- KUWAYAMA Takehito
- Tokyo University of Agriculture, Kanagawa 243-0034, Japan
-
- IWATA Hisataka
- Tokyo University of Agriculture, Kanagawa 243-0034, Japan
この論文をさがす
抄録
The aim of the present study was to examine the fertilization ability and mitochondrial function of oocytes derived from cows with or without liver damage. Oocytes were collected from the ovaries of cows with damaged livers (DL) and those of cows with healthy livers (HL), subjected to in vitro maturation, and fertilized in vitro. A significantly high abnormal fertilization rate was observed for oocytes from DL cows compared to oocytes from HL cows. The time to dissolve the zona pellucida by protease before fertilization was similar between the two liver conditions, whereas after fertilization treatment this time was shorter for DL cows than for HL cows. The percentage of oocytes with equivalent cortical granule distributions underneath the membrane was greater for in vitro matured oocytes from HL cows, whereas an immature distribution pattern was observed for oocytes from DL cows. In addition, a greater percentage of oocytes derived from HL cows released cortical granules following fertilization compared with oocytes from DL cows. Mitochondrial function determined by ATP content and membrane potential were similar at the germinal vesicle stage, but post-in vitro maturation, the oocytes derived from HL cows showed higher values than DL cows. The mitochondrial DNA copy number in oocytes was similar between the two liver conditions for both the germinal vesicle and post-in vitro maturation oocytes. In conclusion, liver damage induces low fertilization, likely because of incomplete cortical granule distribution and release, and the maturation of oocytes from DL cows contain low-functioning mitochondria compared to their HL counterparts.
収録刊行物
-
- Journal of Reproduction and Development
-
Journal of Reproduction and Development 62 (3), 235-240, 2016
公益社団法人 日本繁殖生物学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282681314345472
-
- NII論文ID
- 130005157171
-
- NII書誌ID
- AA10936678
-
- ISSN
- 13484400
- 09168818
-
- NDL書誌ID
- 027429355
-
- PubMed
- 26832309
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可