Growth factor induced proliferation, migration, and lumen formation of rat endometrial epithelial cells <i>in vitro</i>

DOI Web Site Web Site PubMed 39 References
  • ISLAM Md. Rashedul
    Department of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan Department of Genetics and Animal Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh
  • YAMAGAMI Kazuki
    Department of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan
  • YOSHII Yuka
    Department of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan
  • YAMAUCHI Nobuhiko
    Department of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan

Bibliographic Information

Other Title
  • Growth factor induced proliferation, migration, and lumen formation of rat endometrial epithelial cells in vitro

Search this article

Abstract

Endometrial modulation is essential for the preservation of normal uterine physiology, and this modulation is driven by a number of growth factors. The present study investigated the mitogenic, motogenic, and morphogenic effects of epidermal growth factor (EGF) and hepatocyte growth factor (HGF) on rat endometrial epithelial (REE) cells. The REE cells were isolated and cultured and then characterized based on their morphology and their expression of epithelial cell markers. The MTT assay revealed that EGF and HGF induce proliferation of REE cells. Consistent with increased proliferation, we found that the cell cycle regulatory factor Cyclin D1 was also upregulated upon EGF and HGF addition. REE cell migration was prompted by EGF, as observed with the Oris Cell Migration Assay. The morphogenic impact of growth factors on REE cells was studied in a three-dimensional BD Matrigel cell culture system, wherein these growth factors also increased the frequency of lumen formation. In summary, we show that EGF and HGF have a stimulatory effect on REE cells, promoting proliferation, cell migration, and lumen formation. Our findings provide important insights that further the understanding of endometrial regeneration and its regulation.

Journal

References(39)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top