Physiological and Genotypic Characteristics of Nitrous Oxide (N₂O)-Emitting Pseudomonas Species Isolated from Dent Corn Andisol Farmland in Hokkaido, Japan

• Nie Yanxia

  Research Faculty and Graduate School of Agriculture, Hokkaido University

• Li Li

  Research Faculty and Graduate School of Agriculture, Hokkaido University

• Isoda Reika

  Research Faculty and Graduate School of Agriculture, Hokkaido University

• Wang Mengcen

  Research Faculty and Graduate School of Agriculture, Hokkaido University

• Hatano Ryusuke

  Research Faculty and Graduate School of Agriculture, Hokkaido University

• Hashidoko Yasuyuki

  Research Faculty and Graduate School of Agriculture, Hokkaido University

書誌事項

タイトル別名

• Physiological and Genotypic Characteristics of Nitrous Oxide (N₂O)-Emitting <i>Pseudomonas</i> Species Isolated from Dent Corn Andisol Farmland in Hokkaido, Japan

抄録

Dent corn Andisol at the Hokkaido University Shizunai Livestock Experimental Farm actively emits nitrous oxide (N₂O). In order to screen for culturable and active N₂O emitters with high N₂O emission potential, soft gel medium containing excess KNO₃ was inoculated with soil suspensions from farm soil samples collected at different land managements. Dominant bacterial colonies were searched for among 20 of the actively N₂O-emitting cultures from post-harvest soil and 19 from pre-tilled soil, and all isolates were subjected to the culture-based N₂O emission assay. Ten active N₂O-emitting bacteria, four from post-harvest soil and six from pre-tilled soil, out of 156 isolates were identified as genus Pseudomonas by 16S rRNA gene sequencing. These N₂O emitters showed clear responses to NO₃⁻ within a neutral pH range (5.5–6.7), and accelerated N₂O production with 1.5–15 mM sucrose supplementation, suggesting the production of N₂O during the denitrification process. However, the negative responses of 6 active N₂O emitters, 3 from post-harvest soil and 3 from pre-tilled soil, out of the 10 isolates in the acetylene-blocking assay suggest that these 6 N₂O emitters are incomplete denitrifiers that have lost their N₂O reductase (N₂OR) activity. Although the PCR assay for the denitrification-associated genes, narG and nirK/S, was positive in all 10 Pseudomonas isolates, those negative in the acetylene-blocking assay were nosZ-negative. Therefore, these results imply that the high N₂O emission potential of dent corn Andisol is partly attributed to saprophytic, nosZ gene-missing pseudomonad denitrifiers.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 31 (2), 93-103, 2016

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• nitrous oxide (N₂O) emitter
• <i>Pseudomonas</i> sp.
• <i>nosZ</i>-missing and <i>nosZ</i>-harboring denitrifiers
• Andisol corn farm

詳細情報

• CRID: 1390282679323430656
• NII論文ID: 130005158495
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me15155
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 027435675
• PubMed: 27109825
• Web Site: http://id.ndl.go.jp/bib/027435675
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027435675
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/31/2/31_ME15155/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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