<b>Vitamin D</b><b><sub>3</sub></b><b> analog maxacalcitol (OCT) induces hCAP-18/LL-37 production in human oral epithelial </b><b>cells </b>

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Author(s)

    • TADA Hiroyuki
    • Division of Oral Microbiology, Tohoku University Graduate School of Dentistry
    • SHIMIZU Takamitsu
    • Division of Oral Microbiology, Tohoku University Graduate School of Dentistry
    • NAGAOKA Isao
    • Department of Host Defense and Biochemical Research, Juntendo University School of Medicine
    • TAKADA Haruhiko
    • Division of Oral Microbiology, Tohoku University Graduate School of Dentistry

Abstract

Maxacalcitol (22-oxacalcitriol: OCT) is a synthetic vitamin D<sub>3</sub> analog with a limited calcemic effect. In this study, we investigated whether OCT increases the production of LL-37/CAP-18, a human cathelicidin antimicrobial peptide, in human gingival/oral epithelial cells. A human gingival epithelial cell line (Ca9-22) and human oral epithelial cell lines (HSC-2, HSC-3, and HSC-4) exhibited the enhanced expression of LL-37 mRNA upon stimulation with OCT as well as active metabolites of vitamins D<sub>3</sub> and D<sub>2</sub>. Among the human epithelial cell lines, Ca9-22 exhibited the strongest response to these vitamin D-related compounds. OCT induced the higher production of CAP-18 (ng/mL order) until 6 days time-dependently in Ca9-22 cells in culture. The periodontal pathogen <i>Porphyromonas gingivalis</i> was killed by treatment with the LL-37 peptide. These findings suggest that OCT induces the production of hCAP-18/LL-37 in a manner similar to that induced by the active metabolite of vitamin D<sub>3</sub>.

Journal

  • Biomedical Research

    Biomedical Research 37(3), 199-205, 2016

    Biomedical Research Press

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