Reconstruction of a chromatic response system in <i>Escherichia coli</i>
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- Sugie Yoshimi
- Laboratory of Molecular Microbiology, Graduate School of Bioagricultural Sciences, Nagoya University
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- Hori Mayuko
- Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University
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- Oka Shunsuke
- Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University
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- Ohtsuka Hokuto
- Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University
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- Aiba Hirofumi
- Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University
Abstract
Two-component signal transduction systems (TCS) are involved in widespread cellular responses to diverse signals from bacteria to plants. Cyanobacteria have evolved photoperception systems for efficient photosynthesis, and some histidine kinases are known to function as photosensors. In this study, we attempt to reconstruct the photoperception system in Escherichia coli to make an easily controllable ON/OFF switch for gene expressions. For this purpose, a CcaS-CcaR two-component system from Nostoc punctiforme was expressed with phycocyanobilin (PCB) producing enzymes in E. coli which carries a G-box-controlled reporter gene. We succeeded to endow E. coli with a gene activation switch that is regulated in a light-color dependent manner. The possibility of such a switch for the development of synthetic biology is pointed out.
Journal
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 62 (3), 140-143, 2016
Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
