The <i>Arabidopsis</i> CAPRICE protein fused to the VP16 transcriptional activation domain alters root hair and trichome development

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Abstract

<i>CAPRICE</i> (<i>CPC</i>) is a key regulator of epidermal cell fate determination, including root hair and trichome formation, in <i>Arabidopsis thaliana</i>. <i>CPC</i> encodes an R3 MYB transcription factor and is known to be involved in the transcriptional repression of the downstream gene <i>GLABRA2</i> (<i>GL2</i>). We examined transgenic plants harboring CPC fused to a virus-derived transcriptional activator domain (CPC:VP16). Plants carrying <i>35S::CPC:VP16</i> showed increased root hair formation, similar to <i>35S::CPC</i> plants, compared to the wild-type plants. However, transgenic plants harboring <i>CPC:VP16</i> under the control of <i>CPC</i> promoter (<i>CPC::CPC:VP16</i>) showed similar root hair phenotype to that of the wild-type plants, suggesting an inherent cell-to-cell movement ability of CPC:VP16. In this study, all transgenic plants harboring CPC:VP16 possessed a reduced number of trichomes, nearly identical to that of <i>35S::CPC</i> plants, compared to the wild type. Furthermore, we observed some unusual tissues with ectopic trichome clusters in all transgenic plants harboring CPC:VP16. These results indicate that VP16 generally does not confer the transcriptional activation ability to CPC.

Journal

  • Plant Biotechnology

    Plant Biotechnology 33(2), 129-132, 2016

    Japanese Society for Plant Cell and Molecular Biology

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