Cytochrome P450-inhibitory activity of parabens and phthalates used in consumer products

• Ozaki Hitomi

  Graduate School of Biomedical and Health Sciences, Hiroshima University

• Sugihara Kazumi

  Faculty of Pharmaceutical Science, Hiroshima International University

• Watanabe Yoko

  Nihon Pharmaceutical University

• Ohta Shigeru

  Graduate School of Biomedical and Health Sciences, Hiroshima University

• Kitamura Shigeyuki

  Nihon Pharmaceutical University

抄録

The in vitro cytochrome P450 (CYP)-inhibitory effects of 11 parabens and 7 phthalates used in consumer products, as well as their hydrolytic metabolites, were investigated, using rat liver microsomes as an enzyme source. The effects on individual CYP isozymes were evaluated by assaying inhibition of activities towards specific substrates, i.e., ethoxyresorufin O-dealkylase (EROD), methoxyresorufin O-dealkylase (MROD), pentoxyresorufin O-dealkylase (PROD), 7-benzyloxy-4-trifluoromethylcoumarin dealkylase (BFCD), 7-methoxy-4-trifluoromethylcoumarin dealkylase (MFCD) and 7-ethoxy-4-trifluoromethylcoumarin dealkylase (EFCD) activities. These activities were dose-dependently inhibited, most potently by medium-side-chain parabens (C6-9) and phthalates (C4-6), and less potently by shorter- and longer-side-chain esters. The hydrolytic product of parabens, 4-hydroxybenzoic acid, was not inhibitory, while those of phthalates, phthalic acid monoesters, showed lower inhibitory activity than the parent phthalates. Parabens showed relatively potent inhibition of MFCD activity, considered to be mainly due to CYP2C, and phthalates showed relatively potent inhibition of PROD activity, considered to be mainly due to CYP2B.

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ 41 (4), 551-560, 2016

  一般社団法人　日本毒性学会

キーワード

• Consumer product
• Cytochrome P450
• Inhibition
• Paraben
• Phthalate
• Structure-activity relationship

詳細情報

• CRID: 1390282679881718016
• NII論文ID: 130005165024
• NII書誌ID: AN00002808
• DOI: 10.2131/jts.41.551
• ISSN: 18803989
• ISSN: 03881350
• NDL書誌ID: 027587498
• PubMed: 27432241
• Web Site: http://id.ndl.go.jp/bib/027587498
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027587498
• Web Site: https://www.jstage.jst.go.jp/article/jts/41/4/41_551/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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