Protective Effect of Group3 LEA Model Peptides on Enzymes during Desiccation

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Abstract

We examined whether model peptides for group 3 late embryogenesis abundant (G3LEA) proteins that we had developed previously are capable of maintaining enzyme activities under desiccation stress. Two different peptides were tested for comparison. One is PvLEA-22, which consists of two tandem repeats of the 11-mer motif characteristic to G3LEA proteins from African sleeping chironomid. The other is PvLEA-44 made of four tandem repeat of the corresponding 11-mer motif. When lactate dehydrogenase (LDH) is dried without any of the peptides, its enzyme activity is almost lost. However, the LDH activity is preserved 70% or higher in the presence of either the above 22-mer or 44-mer peptide. In addition, the cooperative protective effect is observed when trehalose is added to LDH together with such model peptides before drying. According to these results, we conclude that the G3LEA model peptides can protect enzymes against desiccation stress.

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