Toxic Chemical Cross-linker-free Cryosponges Made from Chitosan-Gluconic Acid Conjugate for Chondrocyte Culture

• Takei Takayuki

  Department of Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University

• Yoshitomi Hiroki

  Department of Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University

• Fukumoto Kohei

  Department of Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University

• Danjo So

  Department of Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University

• Yoshinaga Takuma

  Division of Clinical Application, Nanpuh Hospital

• Nishimata Hiroto

  Department of Gastroenterology, Nanpuh Hospital

• Yoshida Masahiro

  Department of Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University

Abstract

Chitosan has been widely utilized as a porous scaffold material for cartilage regeneration. During the preparation process of chitosan scaffolds, toxic chemical cross-linkers, such as glutaraldehyde, have been used for cross-linking of chitosan molecules. We previously developed chemical cross-linker-free chitosan sponges formed only by freeze-thawing an aqueous solution of chitosan-gluconic acid conjugate (CG). In the present study, we applied the CG cryosponges as scaffolds for cartilage tissue engineering. CG cryosponges with low gluconic acid content showed a defined porous structure and high stability in cell culture medium supplemented with fetal bovine serum that originally contained a low level of lysozyme, a hydrolysis enzyme for chitosan. A favorable mean pore size of the cryosponges for cartilage regeneration (100–300 µm) was accomplished by freezing the CG solution at −30°C. Proliferation and glycosaminoglycan production of bovine chondrocytes were facilitated in plain CG cryosponges compared with those cross-linked with glutaraldehyde. These results demonstrated that the CG cryosponges were promising scaffolds for cartilage tissue engineering.

Journal

• JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

  JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 50 (2), 142-148, 2017

  The Society of Chemical Engineers, Japan

Keywords

• Chitosan
• Cryogel
• Cartilage
• Tissue Engineering
• Glutaraldehyde

Details

• CRID: 1390282679544172800
• NII Article ID: 130005241908
• NII Book ID: AA00709658
• DOI: 10.1252/jcej.16we145
• ISSN: 18811299
• ISSN: 00219592
• NDL BIB ID: 028063724
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028063724
• Web Site: https://www.jstage.jst.go.jp/article/jcej/50/2/50_16we145/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed