<b>Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of </b><b>bone </b>

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  • HANMOTO Taizo
    Noto Marine Laboratory, Division of Marine Environmental Studies, Institute of Nature and Environmental Technology, Kanazawa University
  • TABUCHI Yoshiaki
    Division of Molecular Genetics Research, Life Science Research Center, University of Toyama
  • IKEGAME Mika
    Department of Oral Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ARCOCS, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
  • KONDO Takashi
    Department of Radiological Sciences, Gradu-ate School of Medicine and Pharmaceutical Sciences, University of Toyama
  • KITAMURA Kei-ichiro
    Department of Clini-cal Laboratory Science, Division of Health Sciences, Graduate School of Medical Science, Kanazawa University
  • ENDO Masato
    Department of Marine Biosciences, Tokyo University of Marine Science and Technology
  • KOBAYASHI Isao
    Faculty of Natural System, Institute of Science and Engineering, Kanazawa University
  • MISHIMA Hiroyuki
    Department of Medical Hygiene, Kochi Gakuen College
  • SEKIGUCHI Toshio
    Noto Marine Laboratory, Division of Marine Environmental Studies, Institute of Nature and Environmental Technology, Kanazawa University
  • URATA Makoto
    Noto Marine Laboratory, Division of Marine Environmental Studies, Institute of Nature and Environmental Technology, Kanazawa University Institute of Noto SATOUMI Education and Studies
  • SEKI Azusa
    HAMRI Co. Ltd.
  • YANO Sachiko
    Japan Aerospace Exploration Agency
  • HATTORI Atsuhiko
    Department of Biology, College of Liberal Arts and Sciences, Tokyo Medical and Dental University
  • SUZUKI Nobuo
    Noto Marine Laboratory, Division of Marine Environmental Studies, Institute of Nature and Environmental Technology, Kanazawa University

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  • Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of bone

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Abstract

<p>The effects of low-intensity pulsed ultrasound (LIPUS) on osteoclastogenesis were examined using fish scales that had both osteoclasts and osteoblasts. The binding of the receptor activator of NF-κB ligand (RANKL) in osteoblasts to the receptor activator of NF-κB (RANK) in osteoclasts induced osteoclastogenesis. Therefore, we focused on RANK/RANKL signaling. After 6 h of incubation following LIPUS treatment, mRNA expression of RANKL increased significantly. Resulting from the increased RANKL mRNA level, the expression of transcription-regulating factors significantly increased after 6 h of incubation, and then the mRNA expression of functional genes was significantly up-regulated after 12 h of incubation. However, the mRNA expression of osteoprotegerin (OPG), which is known as an osteoclastogenesis inhibitory factor, also significantly increased after 6 h of incubation and tended to further increase after 12 h of incubation. At 24 h of incubation, osteoclastic functional genes’ mRNA expression decreased to the level of the control. Furthermore, we performed an in vivo experiment with goldfish. Two weeks after daily LIPUS exposure, osteoclastic marker enzymes tended to decrease while osteoblastic marker enzymes were activated. The regeneration rate of the LIPUS-treated scales was significantly higher than that of the control scales. Thus, LIPUS moderately activates osteoclasts and induces bone formation.</p>

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