<b>Hertwig’s epithelial root sheath cells contribute to formation of periodontal ligament through epithelial-mesenchymal transition by </b><b>TGF-β </b>

• ITAYA Satoshi

  Section of Pediatric Dentistry, Department of Oral Growth and Development, Fukuoka Dental College

• OKA Kyoko

  Section of Pediatric Dentistry, Department of Oral Growth and Development, Fukuoka Dental College

• OGATA Kayoko

  Section of Pediatric Dentistry, Department of Oral Growth and Development, Fukuoka Dental College

• TAMURA Shougo

  Section of Pediatric Dentistry, Department of Oral Growth and Development, Fukuoka Dental College

• KIRA -TATSUOKA Michiko

  Section of Pediatric Dentistry, Department of Oral Growth and Development, Fukuoka Dental College

• FUJIWARA Naoki

  Division of Developmental Biology and Regenerative Medicine, Department of Anatomy, Iwate Medical University

• OTSU Keishi

  Division of Developmental Biology and Regenerative Medicine, Department of Anatomy, Iwate Medical University

• TSURUGA Eichi

  Department of Radiation Science, Graduate School of Health Sci-ences, Hirosaki University

• OZAKI Masao

  Section of Pediatric Dentistry, Department of Oral Growth and Development, Fukuoka Dental College

• HARADA Hidemitsu

  Division of Developmental Biology and Regenerative Medicine, Department of Anatomy, Iwate Medical University

Bibliographic Information

Other Title

• Hertwig's epithelial root sheath cells contribute to formation of periodontal ligament through epithelial-mesenchymal transition by TGF-β

Abstract

In tooth root development, periodontal ligament (PDL) and cementum are formed by the coordination with the fragmentation of Hertwig’s epithelial root sheath (HERS) and the differentiation of dental follicle mesenchymal cells. However, the function of the dental epithelial cells after HERS fragmentation in the PDL is not fully understood. Here, we found that TGF-β regulated HERS fragmentation via epithelial-mesenchymal transition (EMT), and the fragmented epithelial cells differentiated into PDL fibroblastic cells with expressing of PDL extracellular matrix (ECM). In the histochemical analysis, TGF-β was expressed in odontoblast layer adjacent of HERS during root development. Periostin expression was detected around fragmented epithelial cells on the root surface, but not in HERS. In the experiment using an established mouse HERS cell line (HERS01a), TGF-β1 treatment decreased E-cadherin and relatively increased N-cadherin expression. TGF-β1 treatment in HERS01a induced further expression of important ECM proteins for acellular cementum and PDL development such as fibronectin and periostin. Taken together, activation of TGF-βsignaling induces HERS fragmentation through EMT and the fragmented HERS cells contribute to formation of PDL and acellular cementum through periostin and fibronectin expression.

Journal

• Biomedical Research

  Biomedical Research 38 (1), 61-69, 2017

  Biomedical Research Press

Keywords

• General Biochemistry, Genetics and Molecular Biology
• General Medicine

Details

• CRID: 1390001204900231808
• NII Article ID: 130005242923
• DOI: 10.2220/biomedres.38.61
• ISSN: 1880313X
• ISSN: 03886107
• PubMed: 28239033
• Web Site: https://www.jstage.jst.go.jp/article/biomedres/38/1/38_61/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018000875
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed